| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L28mt; MAAT1; mrpl28; P15;;MRPL28 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MRPL28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于MRPL28抗体的研究文献摘要(基于公开数据模拟，非真实文献)：
1. **标题**：*MRPL28 is essential for mitochondrial ribosome assembly and respiratory function*
**作者**：Smith J, et al.
**摘要**：本研究利用特异性MRPL28抗体进行免疫印迹和免疫荧光实验，发现MRPL28在线粒体核糖体大亚基组装中起关键作用，敲低该蛋白导致细胞氧化磷酸化功能缺陷。
2. **标题**：*Antibody-based profiling of mitochondrial ribosomal proteins in human cancers*
**作者**：Chen L, et al.
**摘要**：通过开发高特异性MRPL28多克隆抗体，研究团队系统分析了多种癌症中线粒体核糖体蛋白(包括MRPL28)的表达异常，发现其与肿瘤代谢重编程相关。
3. **标题**：*A novel frameshift mutation in MRPL28 causes mitochondrial encephalomyopathy*
**作者**：Wang Y, et al.
**摘要**：利用MRPL28抗体进行患者组织蛋白表达验证，首次报道MRPL28基因移码突变导致线粒体脑肌病的分子机制，揭示了该蛋白在神经系统中的重要作用。
注：上述文献为示例性内容，实际文献需通过PubMed/Google Scholar检索获取。建议使用关键词"MRPL28 antibody"+"mitochondrial ribosome"查询最新研究。
The mitochondrial ribosomal protein L28 (MRPL28) is a component of the large subunit of the mitochondrial ribosome, which plays a critical role in mitochondrial protein synthesis. Mitochondrial ribosomes are essential for translating mitochondrial DNA-encoded mRNAs, particularly those involved in oxidative phosphorylation (OXPHOS), a key pathway for cellular energy production. MRPL28. encoded by the nuclear genome, is imported into mitochondria and assembled into the ribosome structure, contributing to its stability and function.
Antibodies targeting MRPL28 are valuable tools for studying mitochondrial ribosome biogenesis, protein synthesis mechanisms, and mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. These antibodies enable researchers to detect MRPL28 expression levels, assess its subcellular localization via immunofluorescence (IF) or immunohistochemistry (IHC), and investigate interactions with other ribosomal components through co-immunoprecipitation (Co-IP) or Western blotting (WB).
Studies using MRPL28 antibodies have shed light on its potential role in maintaining mitochondrial homeostasis and cellular energy balance. Dysregulation of MRPL28 has been associated with impaired mitochondrial translation, disrupted OXPHOS, and increased reactive oxygen species (ROS), implicating it in pathological conditions. Commercial MRPL28 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown.
Overall, MRPL28 antibodies serve as critical reagents for exploring mitochondrial biology, disease mechanisms, and therapeutic targets, bridging gaps between basic research and clinical applications.
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