首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人notum蛋白同源物(NOTUM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOTUM含量。NOTUM作为一个靶点，它是羧基酯酶家族成员。在研究机制上，其可通过去除Wnt蛋白的棕榈酰基抑制Wnt信号通路。在组织发育、干细胞维持等生理过程以及肿瘤、神经退行性疾病等病理进程中发挥作用，是潜在治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，在组织再生、脂肪代谢及神经退行性疾病研究中具有重要科研价值。适用于探究NOTUM在代谢性疾病、肿瘤微环境调控或神经发育相关机制中的表达变化，为体外实验研究提供可靠的蛋白定量支持，满足基础科研中对Wnt通路相关分子调控机制的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
缩写：NOTUM
Uniprot No.：Q6P988
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-90 | |||||
回收率：
| The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-100 | ||||
| EDTA plasma (n=4) | 106 | 100-114 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6072.4972.5522.378502.2032.0752.1391.965251.6351.5421.5891.41512.50.9521.0060.9790.8056.250.6100.5870.5990.4253.120.4510.4620.4570.2831.560.3320.3150.3240.15000.1760.1710.174 | ||||||
货期：3-5 working days
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