首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠内皮型一氧化氮合成酶(eNOS/NOS3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的NOS3含量。NOS3即内皮型一氧化氮合酶，在血管内皮细胞中表达。它能催化L-精氨酸生成一氧化氮（NO），NO可舒张血管、抑制血小板聚集和白细胞黏附等。研究主要围绕其调节机制及与心血管疾病、肿瘤等发病机制关联展开。试剂盒检测范围为15.6 μIU/mL-1000 μIU/mL，该产品适用于心血管疾病机制研究、内皮功能相关分子通路探索，以及药物干预实验中eNOS表达水平变化的动态监测，例如通过检测不同处理条件下细胞模型或动物模型样本中的eNOS浓度，评估其对血管舒张功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nos3 ELISA Kit; Nitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
缩写：NOS3
Uniprot No.：Q62600
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：15.6 μIU/mL-1000 μIU/mL
灵敏度：3.9 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of rat eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-97 | ||||
| EDTA plasma (n=4) | 94 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/ml.OD1OD2AverageCorrected10002.2032.3042.2542.0775001.9141.9341.9241.7472501.4771.4981.4881.3111250.9530.9510.9520.77562.50.5340.5710.5530.37631.20.3910.3990.3950.21815.60.3170.3250.3210.14400.1750.1780.177 | ||||||
货期：3-5 working days
×
