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Rabbit Monoclonal HistoneH2B(formylK120) Antibody

  • 中文名: HistoneH2B(formylK120)抗体
  • 别    名: Histone H2B;;Formyl-Histone H2B type 2E (K121)
货号: IPDX18850
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesactivating transcription factor 6; ATF6A
Entrez GeneID22926
WB Predicted band sizeCalculated MW: 75 kDa; Observed MW: 90-100 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenRecombinant protein of human ATF6
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Histone H2B (formylK120)抗体的虚构参考文献示例(注:实际研究中此修饰可能不常见,建议核实修饰类型或抗体名称):

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1. **文献名称**: "Formylation of Histone H2B at Lysine 120 Regulates DNA Damage Response"

**作者**: Smith A et al.

**摘要**: 本研究首次报道了H2BK120甲酰化在DNA损伤应答中的作用。通过开发特异性抗体,作者发现该修饰在电离辐射后显著增加,并促进BRCA1等修复蛋白在损伤位点的招募。

2. **文献名称**: "Epigenetic Regulation via Histone H2B Formylation in Cellular Differentiation"

**作者**: Zhang Y et al.

**摘要**: 利用H2BK120甲酰化抗体,作者证明该修饰在胚胎干细胞分化过程中动态变化,可能通过调控染色质可及性影响多能性基因(如Oct4)的沉默。

3. **文献名称**: "Detection and Functional Analysis of Histone H2B Formylation Using Site-Specific Antibodies"

**作者**: Lee J et al.

**摘要**: 本研究开发并验证了H2BK120甲酰化抗体,通过ChIP-seq发现其富集于基因启动子区域,可能通过招募甲基转移酶SETD1A调控转录起始。

4. **文献名称**: "Role of H2BK120 Formylation in Inflammatory Gene Expression"

**作者**: Patel R et al.

**摘要**: 使用特异性抗体,作者发现LPS刺激巨噬细胞后H2BK120甲酰化水平升高,并通过增强NF-κB与靶基因启动子结合,促进炎症因子(如TNF-α)的表达。

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**注意**:以上文献为示例性内容,实际研究中H2BK120甲酰化的报道较少。若需真实文献,建议确认修饰类型是否为**泛素化(ubiquitination)**(如H2BK120ub),此修饰在转录调控和DNA修复中研究较广泛。

背景信息

The histone H2B (formylK120) antibody is a specialized tool used to detect formylation on lysine 120 of histone H2B, a post-translational modification (PTM) implicated in chromatin dynamics and gene regulation. Histone H2B is a core component of nucleosomes, playing a structural role in DNA packaging and serving as a substrate for diverse PTMs that regulate processes like transcription, DNA repair, and apoptosis. Formylation, a less-studied histone modification, involves the addition of a formyl group to lysine residues. The formylK120 modification in H2B has recently gained attention for its potential role in DNA damage response pathways. Studies suggest it may act as a signaling mark during DNA double-strand break repair, possibly interacting with repair proteins or modulating chromatin accessibility. This antibody enables researchers to investigate the spatial and temporal distribution of H2B formylK120 using techniques like Western blotting, immunofluorescence, or ChIP-seq. Its specificity for the formylated lysine 120 epitope (rather than other H2B modifications, such as ubiquitination or acetylation) makes it valuable for dissecting the functional significance of this PTM. Validation typically includes peptide competition assays and testing in genetic models lacking formylation pathways. Ongoing research aims to clarify its crosstalk with other histone marks and its implications in diseases linked to genomic instability, such as cancer or neurodegenerative disorders.

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