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产品描述：人跨膜受体蛋白Notch3(NOTCH3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NOTCH3含量。NOTCH3是一个重要靶点。它参与细胞增殖、分化和凋亡等多个生理过程。研究发现NOTCH3信号通路异常激活与肿瘤、心血管疾病等密切相关，其机制多涉及促进肿瘤细胞生长、血管生成等，深入研究有助于开发针对性治疗药物。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于基础科研领域，如Notch3信号通路调控机制研究、疾病模型中蛋白表达动态分析、药物干预效果评估等实验场景，为心血管疾病、神经系统病变及肿瘤微环境等方向的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CADASIL ELISA Kit; CASIL ELISA Kit; NOTC3_HUMAN ELISA Kit; Notch 3 ELISA Kit; Notch 3 intracellular domain ELISA Kit; Notch homolog 3 ELISA Kit; Notch3 ELISA Kit
缩写：NOTCH3
Uniprot No.：Q9UM47
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NOTCH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of Human NOTCH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-108 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.9132.7172.8152.71040002.2182.3152.2672.16220001.6051.5241.5651.46010000.8840.9230.9040.7995000.5430.5280.5360.4312500.3110.3020.3070.2021250.1820.1920.1870.08200.1060.1040.105 | ||||||
货期：3-5 working days
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