| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 467 |
| clone | 5A9B9 |
| WB Predicted band size | 20.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATF3 (AA: 1-181) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于p66Shc(p66alpha)抗体的参考文献及其摘要概括：
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1. **文献名称**：*"A p66Shc-generated redox signal controls the DNA damage response"*
**作者**：Trine Fiaschi et al.
**摘要**：该研究利用p66Shc特异性抗体，揭示p66Shc通过调控线粒体活性氧(ROS)影响DNA损伤修复的分子机制，发现其缺失会增强细胞对氧化应激的敏感性。
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2. **文献名称**：*"p66Shc is a downstream target of the tumor suppressor BRCA1"*
**作者**：Sandy Chang et al.
**摘要**：通过免疫沉淀和Western blot技术结合p66Shc抗体，证明BRCA1通过调控p66Shc表达抑制氧化应激诱导的细胞凋亡，为乳腺癌治疗提供新靶点。
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3. **文献名称**：*"Deletion of p66Shc gene protects against age-related endothelial dysfunction"*
**作者**：Francesco Paneni et al.
**摘要**：利用p66Shc敲除小鼠模型及抗体检测，发现p66Shc缺失可改善衰老相关的血管内皮功能障碍，提示其作为心血管疾病的潜在治疗靶标。
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注：p66alpha(p66Shc)是Shc家族成员，主要参与氧化应激、衰老及代谢调控。上述文献均通过特异性抗体验证其在疾病中的功能机制。
The p66α antibody is a research tool targeting the p66Shc protein, a 66 kDa isoform encoded by the SHC1 gene. Discovered in the 1990s, p66Shc belongs to the Shc family of adaptor proteins, which also includes p52Shc and p46Shc. Unlike other isoforms, p66Shc contains a unique N-terminal CH2 domain and plays distinct roles in cellular stress responses. It regulates reactive oxygen species (ROS) production, mitochondrial function, and apoptosis, linking oxidative stress to aging and age-related diseases. Studies in knockout mice revealed that p66Shc deficiency extends lifespan and reduces oxidative damage, highlighting its role in aging pathways.
The p66α antibody is widely used in Western blotting, immunohistochemistry, and immunoprecipitation to investigate p66Shc expression, phosphorylation (e.g., at Ser36), and subcellular localization. Its applications span research on metabolic disorders (diabetes, obesity), cardiovascular diseases (atherosclerosis), neurodegenerative conditions (Alzheimer’s), and cancer, where p66Shc modulates ROS-dependent signaling. Additionally, the antibody aids in studying p66Shc’s interaction with proteins like cytochrome c or PKCβ, which mediate its pro-apoptotic functions.
As a key marker in oxidative stress pathways, the p66α antibody remains critical for unraveling molecular mechanisms underlying cellular aging and disease pathogenesis.
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