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产品描述：人N-Myc互作蛋白(NMI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NMI含量。N-Myc互作蛋白(NMI)是一种与NMYC和CMYC相互作用的蛋白质，参与细胞增殖、代谢等生物学过程。NMI与NMYC和CMYC家族成员结合，增强STAT介导的转录，并在多种癌症中表达升高，可能成为癌症治疗的新靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研中探索NMI在肿瘤微环境调控、细胞周期异常或免疫逃逸机制中的功能，也可用于评估实验模型（如基因编辑细胞或动物模型）中NMI的表达动态。为分子机制研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NMI ELISA Kit; N-myc-interactor ELISA Kit; Nmi ELISA Kit; N-myc and STAT interactor ELISA Kit
缩写：NMI
Uniprot No.：Q13287
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 86-92 | ||||||
| 1:4 | Average % | 106 | |||||
| Range % | 103-109 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human NM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 93 | 90-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.6602.7012.6812.5877502.3182.2032.2612.1673751.6761.6981.6871.593187.51.0651.1041.0850.991940.6430.6750.6590.565470.4310.4420.4370.34323.50.2710.2810.2760.18200.0930.0950.094 | |||||||
货期：3-5 working days
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