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产品描述：人NACHT,LRR和PYD结构域蛋白1(NLRP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NLRP1含量。NLRP1是重要的免疫相关靶点。它在固有免疫中发挥关键作用，可感知多种危险信号。其激活后能形成炎性小体，促使炎症因子释放。深入研究其激活、调控等机制，有助于理解免疫疾病发生发展，为相关疾病治疗研究提供新方向。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础研究领域，包括炎症信号通路解析、疾病模型中的蛋白表达动态监测、药物靶点筛选及生物标志物发现等应用场景，配套标准品及显色系统可实现实验室间数据的可靠比对，为先天免疫机制研究提供灵敏的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CARD 7 ELISA Kit; CARD7 ELISA Kit; Caspase recruitment domain protein 7 ELISA Kit; Caspase recruitment domain-containing protein 7 ELISA Kit; CLR17.1 ELISA Kit; Death effector filament forming Ced 4 like apoptosis protein ELISA Kit; Death effector filament-forming ced-4-like apoptosis protein ELISA Kit; DEFCAP ELISA Kit; DEFCAP L/S ELISA Kit; DKFZp586O1822 ELISA Kit; KIAA0926 ELISA Kit; LRR and PYD domains-containing protein 1 ELISA Kit; NAC alpha/beta/gamma/delta ELISA Kit; NAC ELISA Kit; NACHT ELISA Kit; NACHT leucine rich repeat and PYD containing 1 ELISA Kit; NACHT leucine rich repeat and PYD pyrin domain containing 1 ELISA Kit; NACHT leucine rich repeat and pyrin domain containing 1 ELISA Kit; NACHT LRR and PYD containing protein 1 ELISA Kit; NALP 1 ELISA Kit; NALP1 ELISA Kit; NALP1_HUMAN ELISA Kit; NLR family pyrin domain containing 1 ELISA Kit; NLRP 1 ELISA Kit; NLRP1 ELISA Kit; NLRP1 protein ELISA Kit; Nucleotide binding domain and caspase recruitment domain ELISA Kit; Nucleotide binding oligomerization domain leucine rich repeat and pyrin domain containing 1 ELISA Kit; Nucleotide-binding domain and caspase recruitment domain ELISA Kit; PP 1044 ELISA Kit; PP1044 ELISA Kit
缩写：NLRP1
Uniprot No.：Q9C000
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.67 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NLRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-94 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of Human NLRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.0522.1632.1082.0106001.5791.6701.6251.5273000.9840.9780.9810.8831500.5240.5420.5330.435750.3550.3640.3600.26237.50.2620.2520.2570.15918.750.2780.1750.2270.12900.0990.0970.098 | ||||||
货期：3-5 working days
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