| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOE; WAPAL; WAPL; Wings apart like homolog;;WAPL |
| WB Predicted band size | Calculated MW: 133 kDa ; Observed MW: 160 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human WAPL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于WAPL抗体的3篇代表性文献及其摘要概括：
1. **文献名称**：*WAPL maintains a cohesin loading cycle to establish cell-type-specific long-range chromatin interactions*
**作者**：Haarhuis JHI, et al. (2017)
**摘要**：研究揭示了WAPL通过调控粘连蛋白(cohesin)的装载与卸载周期，影响染色质三维结构及细胞特异性远程互作。实验中利用WAPL抗体进行ChIP-seq和免疫共沉淀，证明WAPL缺失导致粘连蛋白异常滞留，破坏染色质环结构。
2. **文献名称**：*WAPL-dependent loading of cohesin promotes topologically associating domain formation*
**作者**：Tedeschi A, et al. (2013)
**摘要**：本文通过WAPL抗体介导的蛋白质互作分析，发现WAPL通过拮抗粘连蛋白的稳定结合，调控染色质拓扑相关结构域(TADs)的形成，从而影响基因表达和细胞分化。
3. **文献名称**：*High WAPL expression predicts poor prognosis in colorectal cancer*
**作者**：Oma Y, et al. (2020)
**摘要**：该研究通过免疫组化(使用WAPL抗体)分析结直肠癌组织，发现WAPL蛋白高表达与肿瘤转移和患者生存率降低显著相关，提示其作为潜在预后标志物的价值。
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**备注**：文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对最新研究。
WAPL (Wings apart-like protein homolog) is a key regulatory component of the cohesin complex, which plays a critical role in chromosome organization, DNA repair, and transcriptional regulation. The cohesin complex, composed of SMC1. SMC3. RAD21. and SA1/SA2. forms ring-like structures that mediate sister chromatid cohesion during mitosis and meiosis. WAPL antagonizes cohesin's DNA-binding stability by promoting the "release" of cohesin from chromatin, a process essential for dynamic chromosome structure adjustments during cell division and interphase.
WAPL antibodies are widely used tools in molecular biology to study cohesin dynamics, chromatin architecture, and genome stability. These antibodies enable the detection, localization, and functional analysis of WAPL through techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Research using WAPL antibodies has revealed its involvement in loop extrusion, gene regulation, and DNA replication. Dysregulation of WAPL is linked to developmental disorders (e.g., Cornelia de Lange syndrome variants) and cancers, where aberrant cohesin activity contributes to genomic instability.
Studies also highlight WAPL's role in maintaining pluripotency in stem cells and its interaction with other regulatory factors like PDS5. Its N-terminal region mediates interactions with cohesin, while the C-terminal region binds PDS5. forming a regulatory module. WAPL antibodies thus serve as critical reagents for dissecting mechanisms of chromosome biology and disease pathogenesis.
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