| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEK6; NimA related protein kinase 6; SID61512;;NEK6 |
| WB Predicted band size | Calculated MW: 36 kDa ; Observed MW: 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NEK6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于NEK6抗体的3篇参考文献及其摘要的简要概括：
---
1. **文献名称**：*"Characterization of a polyclonal antibody against human NEK6 kinase: application in cell cycle regulation studies"*
**作者**：Yin, X.; Liu, W.; et al.
**摘要**：该研究报道了一种针对人源NEK6激酶的多克隆抗体的开发与验证。通过免疫印迹和免疫荧光实验，证明该抗体可特异性识别NEK6蛋白，并发现NEK6在有丝分裂(G2/M期)中调控细胞周期进程，其表达水平与癌细胞增殖相关。
---
2. **文献名称**：*"NEK6 promotes tumor metastasis through modulating EMT and is a therapeutic target in breast cancer"*
**作者**：Lee, S.; Park, T.J.; et al.
**摘要**：研究利用NEK6特异性抗体及shRNA技术，发现NEK6通过激活上皮-间充质转化(EMT)通路促进乳腺癌转移。抑制NEK6表达可显著降低肿瘤侵袭性，提示其作为治疗靶点的潜力。
---
3. **文献名称**：*"Structural insights into NEK6 kinase domain and antibody interaction for targeted drug discovery"*
**作者**：Fry, A.M.; Bayliss, R.; et al.
**摘要**：通过X射线晶体学解析了NEK6激酶结构域的三维结构，并利用特异性抗体研究其功能表位。该研究为基于结构的NEK6抑制剂设计提供了理论基础，并强调抗体在靶向治疗开发中的应用价值。
---
*注：以上文献信息为示例性内容，实际文献需通过PubMed或Google Scholar等平台检索确认。*
The NIMA-related kinase 6 (NEK6) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, particularly during mitosis. NEK6 is involved in spindle formation, chromosome alignment, and cytokinesis, functioning in coordination with other mitotic kinases like Aurora and Polo-like kinases. It is activated during the G2/M phase transition through phosphorylation by upstream regulators, including NEK9. and contributes to the accurate segregation of genetic material. Dysregulation of NEK6 has been linked to various cancers, as its overexpression is observed in tumors such as breast, lung, and colorectal cancers, where it promotes proliferation, survival, and resistance to chemotherapy.
NEK6 antibodies are essential tools for studying its expression, localization, and activity in cellular and disease contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect NEK6 protein levels or post-translational modifications. Specific NEK6 antibodies can distinguish between active (phosphorylated) and inactive forms, aiding in functional studies. Research utilizing NEK6 antibodies has highlighted its role in cancer progression, mitotic errors, and potential as a therapeutic target. Validated antibodies with high specificity are crucial for reliable data, often confirmed through knockout controls or siRNA-based silencing. Understanding NEK6's mechanisms through antibody-based assays continues to advance insights into cell cycle biology and oncogenesis.
×
