首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经丝蛋白H(NF-H)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）样本中的NEFH含量。NEFH即神经丝蛋白重链，是神经元细胞骨架的重要组成部分。它在维持神经元形态、轴突运输等过程中发挥关键作用。研究机制方面，聚焦于其表达异常与神经系统疾病如阿尔茨海默病、帕金森病等的关联，为疾病诊断和治疗提供新思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，主要用于科研领域，支持神经退行性疾病机制研究、药物疗效评估及生物标志物探索，尤其适用于脑脊液等珍贵样本中NF - H的动态变化监测，为神经损伤程度和疾病进展提供量化研究工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：200 kDa neurofilament protein ELISA Kit; CMT2CC ELISA Kit; Nefh ELISA Kit; Neurofilament heavy polypeptide 200kDa ELISA Kit; Neurofilament heavy polypeptide ELISA Kit; Neurofilament triplet H protein ELISA Kit; NF H ELISA Kit; NF-H ELISA Kit; NFH ELISA Kit; NFH_HUMAN ELISA Kit
缩写：NEFH
Uniprot No.：P12036
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF)
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NF-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 84-97 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 95-107 | |||||
回收率：
| The recovery of Human NF-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-90 | ||||
| EDTA plasma (n=4) | 94 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5132.6142.5642.396202.0172.1152.0661.898101.4421.3431.3931.22550.8870.8640.8760.7082.50.5310.5450.5380.3701.250.3740.3620.3680.2000.6250.2760.2840.2800.11200.1670.1680.168 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NF-H antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NF-H antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
