关键信息
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基因名
GTF2H1
- 应用
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别名
GTF2-H1; BTF2; TFIIH; BTF2 p62; Basic transcription factor 2 62 kDa subunit; TFIIH basal transcription factor complex p62 subunit; General Transcription Factor IIH, Polypeptide 1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32780
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表达区间
Thr84~Met335
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分子量
34kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GTF2H1, also known as General Transcription Factor IIH subunit 1, is a pivotal protein involved in the transcription regulation and DNA repair processes in eukaryotic cells. As a core component of the transcription factor IIH (TFIIH) complex, GTF2H1 plays a crucial role in the unwinding of DNA, which is necessary for RNA polymerase II to initiate transcription. Additionally, it participates in nucleotide excision repair, a mechanism for correcting DNA damage caused by environmental factors, such as UV radiation. Mutations or dysregulation of GTF2H1 are associated with various disorders, including xeroderma pigmentosum and Cockayne syndrome, underscoring its importance in maintaining genomic stability. The study and recombinant production of GTF2H1 are essential for elucidating its biochemical properties and functional mechanisms, as well as for developing potential therapeutic strategies for diseases linked to its dysfunction. Understanding the structure and interactions of GTF2H1 may provide insights into the broader context of transcription regulation and DNA repair, facilitating advancements in molecular biology and medicine. Recombination techniques enable the production of this protein in controlled laboratory settings, allowing researchers to investigate its role in cellular processes and assess its potential as a target for drug development. Overall, the exploration of GTF2H1 and its recombinant forms holds significant promise for advancing our comprehension of genetic regulation and the molecular underpinnings of related diseases.
