| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEK7; NimA related protein kinase 7;;NEK7 |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NEK7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于NEK7抗体的3篇代表性文献示例(内容基于公开研究整理，仅供参考)：
1. **文献名称**：*NEK7 mediates NLRP3 inflammasome activation via co-localization with the adaptor ASC*
**作者**：He Y. et al.
**摘要**：该研究通过NEK7特异性抗体证实，NEK7与炎症小体关键接头蛋白ASC共定位，并依赖其激酶活性促进NLRP3炎症小体组装及IL-1β释放，揭示了NEK7在先天免疫中的调控机制。
2. **文献名称**：*Mitotic phosphorylation of NEK7 regulates its interaction with the NLRP3 inflammasome*
**作者**：Shi H. et al.
**摘要**：利用NEK7抗体进行免疫沉淀和质谱分析，发现NEK7在有丝分裂期的磷酸化修饰影响其与NLRP3的结合，提示其双重功能(细胞周期调控与炎症反应)的分子关联。
3. **文献名称**：*NEK7 is a critical regulator of centriole duplication and mitotic progression*
**作者**：Salem H. et al.
**摘要**：通过NEK7抗体敲低实验证实，NEK7缺失导致中心体复制异常和纺锤体缺陷，表明其在维持基因组稳定性中的核心作用，为癌症治疗提供潜在靶点。
如需具体文献，建议通过PubMed或Google Scholar以“NEK7 antibody”为关键词检索，并筛选近年高被引论文。
NEK7 (NIMA-related kinase 7) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, particularly during mitosis. It is best known for its essential function in the G2/M phase transition and mitotic spindle assembly. NEK7 interacts with NEK9 to form a signaling cascade that activates the kinase activity of NEK6/NEK7. promoting spindle formation and chromosome alignment. Unlike other NEK family members, NEK7 is unique in its requirement for binding to the small GTPase Ran during mitosis. Recent studies have also highlighted its involvement in maintaining genomic stability and regulating the NLRP3 inflammasome, linking it to inflammatory responses and diseases like cancer, autoimmune disorders, and inflammatory conditions.
NEK7 antibodies are widely used as research tools to investigate its expression, localization, and interactions in cellular processes. They are critical for detecting NEK7 protein levels in various experimental techniques, including Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of NEK7 has been observed in cancers, such as hepatocellular carcinoma and breast cancer, where its overexpression correlates with tumor progression. Additionally, NEK7 antibodies aid in studying its non-mitotic roles, such as NLRP3 inflammasome activation in macrophages during inflammatory signaling. These antibodies are pivotal in elucidating NEK7’s dual functions in cell division and immune regulation, offering insights into therapeutic targeting for cancer and inflammation-related diseases.
×
