首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人内脂素/内脏脂肪素(visfatin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的NAMPT含量。NAMPT（烟酰胺磷酸核糖转移酶）是NAD+生物合成途径的关键限速酶，参与能量代谢和细胞存活。其在肿瘤细胞中表达升高，与肿瘤发生发展密切相关。研究NAMPT机制，有助于开发针对肿瘤的新药。试剂盒检测范围为0.625 ng/mL-40 ng/mL，科研人员可通过探究 visfatin 在代谢性疾病动物模型中的表达动态，分析临床样本与病理进程的关联性，或用于药物干预实验的分子效应评估，为肥胖相关机制研究、新型治疗靶点开发等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1110035O14Rik ELISA Kit; AI314458 ELISA Kit; AI480535 ELISA Kit; DKFZP666B131 ELISA Kit; EC 2.4.2.12 ELISA Kit; MGC117256 ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; NAMPT_HUMAN ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; PBEF 1 ELISA Kit; PBEF ELISA Kit; PBEF1 ELISA Kit; Pre B cell colony enhancing factor 1 ELISA Kit; Pre B cell colony enhancing factor ELISA Kit; Pre B cell enhancing factor ELISA Kit; Pre-B cell-enhancing factor ELISA Kit; Pre-B-cell colony-enhancing factor 1 ELISA Kit; VF ELISA Kit; Visfatin ELISA Kit
缩写：NAMPT
Uniprot No.：P43490
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of human visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-104 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected401.9772.0502.0141.921201.5261.5841.5551.462101.0871.1011.0941.00150.6580.7030.6810.5882.50.4510.4210.4360.3431.250.2930.3010.2970.2040.6250.1650.1590.1620.06900.0960.0890.093 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NAMPT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NAMPT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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