首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肌抑素(MSTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MSTN含量。MSTN（肌肉生长抑制素）是转化生长因子β家族成员，主要调节骨骼肌生长和发育。研究显示，MSTN缺失可促进骨骼肌生长，并改变肠道微生物群组成，其机制可能与肠道微生物代谢产物戊酸通过GPR43激活Akt/mTOR信号通路有关。试剂盒检测范围为0.625 ng/mL-20 ng/mL，该产品适用于探究肌肉发育机制、运动代谢调控、肌肉萎缩相关疾病模型等基础研究领域，尤其可为运动科学、肌肉再生及代谢性疾病（如肥胖、Ⅱ型糖尿病）的分子机制研究提供可靠检测工具。通过检测不同生理或病理条件下生物样本中MSTN的浓度变化，研究人员可深入解析其在肌肉 - 脂肪代谢网络中的动态调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cmpt ELISA Kit; GDF-8 ELISA Kit; GDF8_HUMAN ELISA Kit; Growth/differentiation factor 8 ELISA Kit; MSLHP ELISA Kit; MSTN ELISA Kit; MSTN myostatin ELISA Kit; Myostatin ELISA Kit; Myostatin Propeptide ELISA Kit
缩写：MSTN
Uniprot No.：O14793
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-20 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-90 | |||||
回收率：
| The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 86 | 82-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 201.8511.9921.9221.878 101.0581.1361.0971.053 4.3750.5020.5240.5130.469 1.8750.2680.2550.2620.218 0.6250.1160.1190.1180.074 00.0430.0450.044 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MSTN antibody.
Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated MSTN antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
Substrate A (1 x 7 ml)
Substrate B (1 x 7 ml)
One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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