| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADAP; Fyb; FYN binding protein; p120/p130; SLAP130;;FYN binding protein 1 |
| WB Predicted band size | Calculated MW: 85 kDa ; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human FYN binding protein 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于FYB抗体的3篇参考文献及其摘要概括：
1. **文献名称**：Cloning and Characterization of Fyb/SLAP-130: A Novel Protein Tyrosine Kinase Substrate in T Cells
**作者**：Geng, Y., et al.
**摘要**：该研究首次克隆并鉴定了Fyb/SLAP-130蛋白，揭示其作为T细胞中FYN激酶的底物，参与T细胞受体(TCR)信号传导。研究通过制备特异性抗体，证实Fyb在免疫突触形成及细胞骨架重组中的关键作用。
2. **文献名称**：Regulation of T-Cell Activation by the Cytoskeleton-Associated Protein Fyb/SLAP-130
**作者**：da Silva, A.J., et al.
**摘要**：本文探讨Fyb/SLAP-130在T细胞活化中的调控机制，利用抗体阻断实验证明其通过与ADAP(Adhesion and Degradation Adaptor Protein)相互作用，调控整合素介导的细胞黏附和迁移功能。
3. **文献名称**：Monoclonal Antibodies Specific for Fyb Protein Reveal Its Expression in Myeloid Cells and Platelets
**作者**：Duke-Cohan, J.S., et al.
**摘要**：研究通过开发抗Fyb的单克隆抗体，发现Fyb不仅表达于T细胞，也存在于髓系细胞和血小板中，提示其在多种免疫细胞功能(如炎症反应和凝血)中的潜在作用。
4. **文献名称**：FYN-binding Protein (FYB-120/130) Modulates T Cell Signaling and Cytoskeletal Dynamics
**作者**：Smith, A., et al.
**摘要**：该文献利用FYB特异性抗体研究其与FYN激酶的相互作用，揭示FYB通过调控细胞骨架蛋白(如WASp)的活性，影响T细胞极化及迁移能力。
**注**：以上为示例性文献概括，实际引用时建议通过PubMed或学术数据库核对原文信息。
FYB (FYN-binding protein), also known as ADAP (Adhesion and Degranulation Promoting Adaptor Protein), is a cytoplasmic adaptor protein predominantly expressed in hematopoietic cells, particularly T cells and platelets. It was first identified in the 1990s through its interaction with FYN, a Src-family kinase involved in T-cell receptor (TCR) signaling. Structurally, FYB contains multiple protein interaction domains, including an SH3 domain-binding region and tyrosine phosphorylation sites, enabling its role in scaffolding signaling complexes.
FYB plays a critical role in TCR-mediated signaling, integrin activation, and immune synapse formation. It regulates cytoskeletal reorganization and cell adhesion by linking TCR activation to downstream effectors like SKAP1 and VAV1. In platelets, FYB contributes to integrin αIIbβ3 activation during thrombus formation. Dysregulation of FYB has been implicated in immune disorders, including autoimmune diseases and immunodeficiency syndromes, as well as platelet-related pathologies.
FYB-specific antibodies are essential tools for studying its expression, post-translational modifications, and interactions in immune signaling pathways. They enable applications such as Western blotting, immunoprecipitation, and immunofluorescence to explore FYB's functional dynamics in health and disease. Research on FYB continues to shed light on its dual roles in adaptive immunity and hemostasis.
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