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产品描述：人组织相容性复合体Ⅰ类相关基因A(MICA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MICA含量。MICA为一种靶点。其在肿瘤免疫等领域有重要背景，可作为免疫治疗的潜在靶点。机制上，MICA能与NKG2D受体结合激活免疫细胞，增强免疫细胞对肿瘤细胞的杀伤作用，为肿瘤免疫治疗研究提供了新的方向和思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于基础科研中探究MICA在肿瘤微环境、感染性疾病或炎症反应中的表达动态，以及评估药物干预对MICA调控机制的影响，为免疫相关疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MHC class I chain-related protein A ELISA Kit; MHC class I chain-related protein B ELISA Kit; MHC class I polypeptide related sequence A ELISA Kit; MHC class I polypeptide related sequence B ELISA Kit; MHC class I polypeptide-related sequence A ELISA Kit; MIC-A ELISA Kit; micA ELISA Kit; MICA_HUMAN ELISA Kit; MICB ELISA Kit
缩写：MICA
Uniprot No.：Q29983
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MICA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 83-95 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 84-96 | ||||||
回收率：
| The recovery of human MICA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-98 | |||||
| EDTA plasma (n=4) | 106 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1002.5892.6882.6392.562502.1482.3222.2352.158251.4391.4771.4581.38112.50.7780.8210.8000.7236.250.4130.4330.4230.3463.120.2640.2560.2600.1831.560.1650.1660.1660.08900.0750.0780.077 | |||||||
货期：3-5 working days
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