首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白血病抑制因子(LIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、支气管肺泡灌洗液、组织匀浆样本中的LIF含量。LIF（白血病抑制因子）是一种多效性细胞因子，属于IL-6家族，参与细胞生长、分化和免疫反应。其研究机制涉及调节细胞增殖、分化和表型，影响骨代谢、神经发育等。LIF通过与其受体结合，激活JAK/STAT和MAPK信号通路，参与多种疾病的发病过程。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于呼吸系统疾病模型研究、干细胞分化调控实验及炎症相关机制探索，可为肺纤维化、急性肺损伤等动物模型中的LIF动态监测提供可靠工具，助力科研人员深入解析LIF在组织再生、免疫微环境调节中的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
缩写：LIF
Uniprot No.：P17777
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-92 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.2492.1742.2122.039101.5831.5611.5721.39951.0120.9520.9820.8092.50.5990.5830.5910.4181.250.4460.4160.4310.2580.6250.3200.2990.3100.1370.3120.2580.2450.2520.07900.1770.1690.173 | ||||||
货期：3-5 working days
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