首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠黄体生成素（LH）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的LH含量。LH即促黄体生成素，是腺垂体分泌的糖蛋白激素。它在生殖系统中扮演重要角色，可刺激卵巢排卵和黄体形成，促进雄激素合成与分泌。研究表明，其水平变化与多种生殖内分泌疾病相关，深入研究其作用机制有助于疾病诊断与治疗。试剂盒检测范围为0.3 mlU/ml-60 mlU/ml，适用于科研领域探索生殖系统疾病模型建立、动情周期激素动态监测、药物干预对性腺轴的影响等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
缩写：LH
Uniprot No.：P01230
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.3 mlU/ml-60 mlU/ml
灵敏度：0.15 mlU/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 85-105 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-107 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average 600.1500.1530.152 150.3520.3380.345 4.80.5130.5490.531 1.20.9851.0020.994 0.31.4761.4411.459 02.6792.6542.667 | ||||||
货期：3-5 working days
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