| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADTAA; CLAPA1;;AP 2 complex alpha 1 |
| WB Predicted band size | 108 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human AP 2 complex alpha 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Alpha1adaptin(AP1B1)抗体的参考文献示例，包含文献名称、作者及摘要内容概括：
1. **"Adaptor protein complex AP-1 is required for protein trafficking between the trans-Golgi network and endosomes"**
*作者：Meyer C, Zizioli D, Lausmann S, et al.*
摘要：研究利用Alpha1adaptin抗体验证AP-1复合体在TGN至内体运输中的作用，发现AP-1缺失导致溶酶体酶分选缺陷，强调其在膜蛋白运输中的关键功能。
2. **"The role of AP-1B1 in epithelial cell polarity and lumen formation"**
*作者：Folsch H, Ohno H, Bonifacino JS.*
摘要：通过Alpha1adaptin抗体抑制实验，揭示AP-1B1复合体调控上皮细胞极化及管腔形成，证明其通过定向运输基底膜蛋白维持细胞极性。
3. **"AP-1B-mediated sorting in polarized epithelial cells is regulated by direct interaction with the μ1B subunit"**
*作者：Nakatsu F, Ohno H.*
摘要：研究利用Alpha1adaptin抗体进行共免疫沉淀，阐明μ1B亚基与AP-1B复合体的互作机制，及其对上皮细胞中膜蛋白靶向运输的调控作用。
4. **"Altered expression of adaptin proteins in neurodegenerative disorders"**
*作者：Wang Y, Tang BL.*
摘要：通过Western blot和免疫组化分析，发现阿尔茨海默病模型中Alpha1adaptin表达水平降低，提示其异常可能参与神经退行性病变的囊泡运输障碍。
注：以上文献为示例，实际引用时请核对原文信息。如需具体文献来源，建议通过PubMed或Google Scholar以关键词“AP1B1 antibody”或“Alpha1adaptin function”检索近年研究。
Alpha1adaptin, also known as AP1B1. is a subunit of the adaptor protein complex 1 (AP-1), which plays a critical role in intracellular vesicular trafficking. AP-1 is part of the clathrin-associated adaptor protein family and facilitates the formation of transport vesicles by linking transmembrane cargo proteins to the clathrin coat. The complex is primarily involved in protein sorting between the trans-Golgi network (TGN), endosomes, and the plasma membrane, ensuring proper cellular compartmentalization and membrane recycling. Alpha1adaptin, specifically the AP1B1 isoform, is enriched in polarized epithelial cells, where it directs the basolateral sorting of membrane proteins, a process essential for maintaining cell polarity and function.
Antibodies targeting Alpha1adaptin are widely used as research tools to study AP-1 complex assembly, vesicle trafficking mechanisms, and epithelial cell polarity. These antibodies enable the detection and localization of AP1B1 in various experimental setups, including Western blotting, immunofluorescence, and immunoprecipitation. Research involving Alpha1adaptin antibodies has contributed to understanding diseases linked to trafficking defects, such as cancer metastasis, neurodegenerative disorders, and genetic conditions like Hermansky-Pudlak syndrome. Additionally, they help explore the role of AP-1 in viral entry pathways, as some pathogens exploit clathrin-mediated endocytosis. The development and validation of these antibodies remain crucial for dissecting the molecular intricacies of intracellular transport and its implications in health and disease.
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