| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CP27; CTX; CYP; CYP27; CYP27A1;;Cytochrome P450 27A1 |
| WB Predicted band size | Calculated MW: 60 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 27A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于CYP27A1抗体的3篇参考文献示例(内容基于虚构摘要，仅供格式参考)：
1. **文献名称**: *"CYP27A1 expression and localization in human liver: Role in bile acid biosynthesis"*
**作者**: Björkhem I, et al.
**摘要**: 通过Western blot和免疫组化分析，利用CYP27A1特异性抗体证实其在肝细胞线粒体中的表达，研究其与胆汁酸合成途径的关联。
2. **文献名称**: *"Antibody-based detection of CYP27A1 mutations in cerebrotendinous xanthomatosis patients"*
**作者**: Calvo PL, García SY.
**摘要**: 采用CYP27A1多克隆抗体对CTX患者肝组织进行免疫印迹分析，揭示突变导致蛋白表达缺失或功能异常，支持基因诊断结果。
3. **文献名称**: *"CYP27A1 enzyme activity modulation by environmental toxins: Insights from antibody-mediated quantification"*
**作者**: Hsu MH, et al.
**摘要**: 使用CYP27A1单克隆抗体研究环境毒素暴露后酶蛋白水平变化，发现其表达下调可能加剧胆固醇代谢紊乱。
注：以上文献为示例，实际引用需检索PubMed等数据库获取真实文献(如搜索关键词：CYP27A1 antibody, Western blot, immunohistochemistry)。
The CYP27A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 27 subfamily A member 1 (CYP27A1), a mitochondrial protein involved in cholesterol metabolism and bile acid synthesis. CYP27A1 catalyzes the hydroxylation of cholesterol to form 27-hydroxycholesterol, a key step in the alternative pathway of bile acid production. It also plays a role in vitamin D3 activation by converting vitamin D3 to 25-hydroxyvitamin D3. Mutations in the CYP27A1 gene are linked to cerebrotendinous xanthomatosis (CTX), a rare autosomal recessive disorder characterized by abnormal cholesterol and cholestanol accumulation in tissues, leading to neurological, ocular, and cardiovascular complications.
CYP27A1 antibodies are widely used in research to detect protein expression, localization, and regulation in tissues such as the liver, brain, and vascular endothelium. These antibodies enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate CYP27A1's role in metabolic pathways, disease mechanisms, and potential therapeutic targets. Polyclonal and monoclonal variants are available, often validated for specificity in human, mouse, or rat models. Studies using these antibodies have clarified CYP27A1's involvement in atherosclerosis, neurodegenerative diseases, and cancer, highlighting its dual role in lipid homeostasis and inflammation. Reliable detection of CYP27A1 aids in diagnosing CTX and understanding its pathophysiology.
×
