| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAP12; KARAP; PLOSL; TYROBP;;DAP12 |
| WB Predicted band size | 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human DAP12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DAP12抗体的3篇代表性文献的简要信息：
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1. **文献名称**: *DAP12-mediated signal transduction in natural killer cells*
**作者**: Lanier LL, et al.
**摘要**: 本文研究了DAP12在NK细胞激活中的作用，利用特异性抗体阻断DAP12信号通路，发现其通过结合KIR家族受体调控细胞毒性及细胞因子分泌，揭示DAP12在先天免疫中的关键功能。
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2. **文献名称**: *TREM2-DAP12 signaling in neurodegenerative diseases*
**作者**: Colonna M, et al.
**摘要**: 通过抗DAP12抗体阻断实验，作者阐明DAP12与TREM2受体互作在小胶质细胞中的机制，并发现该通路异常与阿尔茨海默病等神经退行性疾病中的炎症反应和病理沉积相关。
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3. **文献名称**: *Structural basis for DAP12 assembly with activating receptors*
**作者**: Feng J, et al.
**摘要**: 利用抗DAP12抗体进行免疫共沉淀和结构分析，揭示DAP12与免疫受体(如NKG2D)结合的分子构象，为理解其跨膜信号转导及免疫突触形成提供结构生物学证据。
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以上文献均聚焦于DAP12抗体的功能研究或实验应用，涵盖免疫调控、疾病机制和结构解析等领域。如需具体期刊或年份信息，可进一步补充检索。
DAP12 (DNAX-activating protein 12), also known as KARAP or TYROBP, is a transmembrane adaptor protein critical for signaling in immune cells. It belongs to the immunoglobulin superfamily and contains a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). DAP12 pairs with cell-surface receptors lacking intrinsic signaling domains, such as TREM (triggering receptor expressed on myeloid cells) and NK cell receptors, enabling signal transduction upon ligand binding. Upon receptor engagement, DAP12 undergoes phosphorylation at its ITAM, recruiting Syk or ZAP70 kinases to initiate downstream pathways that regulate cellular activation, cytokine production, and cytotoxicity.
DAP12 is primarily expressed in natural killer (NK) cells, macrophages, dendritic cells, and microglia. Its dysfunction is linked to diseases like Nasu-Hakola disease, a rare genetic disorder characterized by bone cysts and early-onset dementia due to DAP12 or TREM2 mutations. Aberrant DAP12 signaling is also implicated in neurodegenerative conditions (e.g., Alzheimer’s) and autoimmune disorders.
DAP12-specific antibodies are essential research tools for studying receptor interactions, signaling mechanisms, and disease pathogenesis. They enable detection of DAP12 expression, block functional activity, or assess post-translational modifications. Therapeutic antibodies targeting DAP12-associated pathways are under exploration for modulating immune responses in inflammatory and neurological diseases.
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