| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK 3; JTK5; JTK5A; Ryk; RYK1; Vik;;RYK |
| WB Predicted band size | Calculated MW: 68 kDa ; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RYK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RYK抗体的3篇参考文献，简要整理如下：
1. **文献名称**: "The RYK receptor is required for Wnt5a-mediated axon guidance in the mammalian forebrain"
**作者**: Yoshikawa et al. (2003)
**摘要**: 该研究揭示了RYK受体在哺乳动物前脑轴突导向中的关键作用，通过结合Wnt5a信号调控神经轴突生长。研究中使用特异性RYK抗体进行免疫组化分析，证实RYK在神经元中的表达模式及其与Wnt信号通路的关联。
2. **文献名称**: "RYK is a novel Wnt receptor regulating cancer stem cell activity in non-small cell lung cancer"
**作者**: Katoh et al. (2002)
**摘要**: 本文探讨RYK作为非典型Wnt受体在非小细胞肺癌干细胞中的功能。通过Western blot和免疫荧光实验(使用RYK抗体)，研究发现RYK通过调控β-catenin非依赖性信号通路促进肿瘤干细胞的自我更新和侵袭。
3. **文献名称**: "Ryk regulates neuronal survival through interaction with the Alzheimer’s amyloid precursor protein"
**作者**: Estevez et al. (2017)
**摘要**: 该研究报道RYK与阿尔茨海默病淀粉样前体蛋白(APP)的相互作用，影响神经元存活。利用RYK特异性抗体进行共免疫沉淀实验，发现RYK-APP复合物通过调控突触功能参与神经退行性病变。
4. **文献名称**: "RYK mediates Wnt cell signaling and is required for mammalian tissue patterning during development"
**作者**: Macheda et al. (2012)
**摘要**: 研究通过敲除模型和RYK抗体标记，证明RYK在胚胎发育过程中介导Wnt信号，参与多种组织(如骨骼和神经系统)的形态发生和细胞命运决定。
以上文献均涉及RYK抗体的实验应用，涵盖神经发育、癌症和疾病机制等领域。
RYK (Receptor-like Tyrosine Kinase) is an atypical member of the receptor tyrosine kinase (RTK) family, distinguished by its catalytically inactive pseudokinase domain. Initially identified in *Drosophila* as a regulator of embryonic patterning, RYK functions primarily as a co-receptor in Wnt signaling pathways, particularly the planar cell polarity (PCP) and β-catenin-independent pathways. Its extracellular domain contains leucine-rich repeats and a Wnt-inhibitory factor (WIF) motif, enabling interactions with Wnt ligands like Wnt5a. Intracellularly, RYK recruits signaling mediators such as Dishevelled (Dvl) through its PDZ-binding domain.
RYK is critical in embryonic development, influencing axon guidance, neural crest migration, and limb morphogenesis. Dysregulation of RYK signaling is implicated in cancer, neurodevelopmental disorders, and neurodegenerative diseases. For instance, RYK overexpression in glioblastoma and breast cancer promotes invasiveness, while its loss contributes to neural tube defects.
RYK antibodies are essential tools for studying its expression, localization, and interactions. They are used in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to dissect RYK's role in health and disease. Monoclonal antibodies targeting specific epitopes aid in functional studies, while phospho-specific antibodies help map signaling events. Challenges remain in distinguishing RYK isoforms and minimizing cross-reactivity due to structural similarities with other RTKs. Ongoing research focuses on developing therapeutic antibodies to modulate RYK activity in pathological contexts.
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