| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ESP2; HED2; ZYX; Zyxin2;;Zyxin |
| WB Predicted band size | Calculated MW: 61 kDa ; Observed MW: 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Zyxin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Zyxin抗体的代表性文献摘要(文献信息为示例性质，实际引用时需核实)：
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1. **标题**: "Zyxin regulates cell migration and actin filament dynamics through its interaction with α-actinin"
**作者**: Drees, B.E., et al.
**摘要**: 本研究利用Zyxin特异性抗体进行免疫荧光定位，发现Zyxin与α-actinin在细胞迁移前沿共定位，并通过调控肌动蛋白重塑参与细胞运动。抗体实验证实Zyxin缺失导致细胞迁移能力下降，揭示了其在细胞骨架动态中的关键作用。
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2. **标题**: "Zyxin antibody-based analysis of focal adhesion turnover in cancer metastasis"
**作者**: Smith, J.R., & Chen, L.
**摘要**: 通过Western blot和免疫组化使用Zyxin抗体，研究发现转移性癌细胞中Zyxin表达显著上调，且其与黏着斑(focal adhesion)分解相关。提示Zyxin可能作为癌症转移的生物标志物及潜在治疗靶点。
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3. **标题**: "A functional study of Zyxin in zebrafish embryogenesis using antibody-mediated knockdown"
**作者**: Tanaka, H., et al.
**摘要**: 采用Zyxin抗体进行斑马鱼胚胎显微注射，发现Zyxin功能抑制导致胚胎体节发育异常，证明其通过调控细胞-基质黏附参与胚胎形态发生，为发育生物学研究提供新工具。
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4. **标题**: "Proteomic identification of Zyxin-interacting proteins in mechanotransduction pathways"
**作者**: Gupta, M., et al.
**摘要**: 基于Zyxin抗体的免疫共沉淀(Co-IP)结合质谱分析，鉴定出Zyxin与VASP、LASP1等机械敏感蛋白的相互作用，揭示了其在细胞力学信号传导中的分子网络。
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**提示**：实际文献检索建议通过PubMed或Google Scholar以关键词“Zyxin antibody” + 研究领域(如“cancer”“mechanotransduction”)筛选近5年高引论文，并优先选择《Journal of Cell Biology》《Nature Cell Biology》等权威期刊。
Zyxin is a cytoskeletal protein primarily localized at focal adhesions and cell-cell junctions, playing critical roles in cell adhesion, mechanotransduction, and actin cytoskeleton remodeling. It regulates cell motility by shuttling between adhesion sites and the nucleus, influencing gene expression and stress fiber assembly. Zyxin contains LIM domains that mediate protein-protein interactions, enabling its involvement in signaling pathways responsive to mechanical forces.
Zyxin antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate zyxin's role in wound healing, cancer metastasis, and cardiovascular development. Researchers also employ these antibodies to explore zyxin's interaction with partners such as α-actinin, VASP, and members of the Ena/VASP family.
Commercial zyxin antibodies are typically raised in rabbits or mice against specific epitopes, often within its N-terminal proline-rich region or C-terminal LIM domains. Validation includes testing in knockout cell lines or tissues to confirm specificity. Dysregulation of zyxin has been linked to pathological conditions like fibrosis and tumor progression, underscoring the antibody's relevance in both basic research and clinical applications.
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