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产品描述：人层粘连蛋白亚基α-3(LAMA3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LAMA3含量。LAMA3 是一个重要靶点。它在细胞外基质中发挥关键作用，与细胞黏附、迁移等过程密切相关。研究聚焦其在多种疾病如癌症中的机制，探索其表达变化对肿瘤进展、转移的影响，以期为开发针对性的治疗策略提供依据。试剂盒检测范围为9.4 ng/mL-600 ng/mL，适用于科研领域中对LAMA3表达水平的定量分析，例如研究皮肤疾病模型中的基底膜损伤机制、肿瘤微环境中细胞外基质重塑过程，或通过组织样本评估LAMA3在特定生理病理状态下的动态变化。可为细胞生物学、分子病理学等研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：E170 ELISA Kit; Epiligrin 170 kDa subunit ELISA Kit; Epiligrin subunit alpha ELISA Kit; Kalinin B1 chain ELISA Kit; Kalinin subunit alpha ELISA Kit; LAMA3 ELISA Kit; LAMA3_HUMAN ELISA Kit; LAMB3 ELISA Kit; LAMC2 ELISA Kit; Laminin gamma 2 ELISA Kit; Laminin 5 beta 3 ELISA Kit; Laminin 5 gamma 2 subunit ELISA Kit; Laminin beta 3 ELISA Kit; Laminin subunit alpha-3 ELISA Kit; Laminin subunit beta 3 ELISA Kit; Laminin subunit gamma 2 ELISA Kit; Laminin-5 subunit alpha ELISA Kit; Laminin-6 subunit alpha ELISA Kit; Laminin-7 subunit alpha ELISA Kit; Nicein subunit alpha ELISA Kit
缩写：LAMA3
Uniprot No.：Q16787
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：9.4 ng/mL-600 ng/mL
灵敏度：2.35 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 97 | |||||
| Range % | 89-101 | ||||||
| 1:10 | Average % | 106 | |||||
| Range % | 100-110 | ||||||
| 1:20 | Average % | 93 | |||||
| Range % | 86-96 | ||||||
| 1:40 | Average % | 107 | |||||
| Range % | 92-111 | ||||||
回收率：
| The recovery of human LAMA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 95-107 | |||||
| EDTA plasma (n=4) | 104 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected6002.4922.5662.5292.3553001.7431.7081.7261.5521501.1691.1241.1470.973750.7620.7450.7540.58037.50.4400.4670.4540.28018.750.3230.3330.3280.1549.40.2440.2510.2480.07400.1720.1750.174 | |||||||
货期：3-5 working days
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