| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p53DINP1; SIP; Teap; TP53DINP1; TP53INP1; TP53INP1A; TP53INP1B;;p53 DINP1 |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human p53 DINP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是假设的3条关于p53/DINP1抗体的参考文献示例(注：实际文献可能需要根据具体数据库检索，以下为模拟内容)：
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1. **文献名称**: *DINP1 regulates p53-mediated apoptosis through a novel feedback loop*
**作者**: Tanaka K. et al.
**摘要**: 本研究利用特异性DINP1抗体，揭示了DINP1在DNA损伤后通过稳定p53蛋白促进细胞凋亡的机制。实验表明，DINP1抗体通过免疫共沉淀证实其与p53的直接相互作用，并发现DINP1缺失会削弱p53的转录活性。
2. **文献名称**: *Development of a monoclonal antibody against human DINP1 for cancer diagnostics*
**作者**: Chen L. et al.
**摘要**: 文章报道了一种新型抗人DINP1单克隆抗体的开发，验证了其在免疫组化中的特异性。研究发现，DINP1在结直肠癌中高表达且与p53突变状态相关，提示其作为生物标志物的潜力。
3. **文献名称**: *p53-dependent induction of DINP1 under oxidative stress*
**作者**: Müller S. et al.
**摘要**: 通过Western blot和ChIP实验(使用p53和DINP1抗体)，作者证明氧化应激下p53直接结合DINP1启动子并激活其表达，DINP1可能通过调控ROS水平影响细胞命运。
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**注意事项**：
- 实际文献需通过PubMed、Google Scholar等平台以关键词“DINP1 antibody”“p53 DINP1 interaction”检索。
- 若研究较少，可扩展至DINP1功能或p53通路相关抗体研究。
The p53DINP1 (p53-dependent damage-inducible nuclear protein 1) antibody is a tool used to study the p53 signaling pathway, which plays a central role in maintaining genomic stability and tumor suppression. p53DINP1. also known as CR673673 or C20orf43. is a stress-responsive protein transcriptionally activated by p53 under DNA damage or oxidative stress. It participates in cell cycle arrest, DNA repair, and apoptosis by interacting with key regulators like ATM/ATR kinases and modulating p53 activity through post-translational modifications.
The antibody specifically detects endogenous or overexpressed p53DINP1 protein in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its development aids in elucidating the protein's role in stress response, cancer biology, and aging. Researchers use it to analyze p53DINP1 expression patterns in normal versus tumor tissues, assess its regulation during therapeutic interventions (e.g., chemotherapy or radiation), and explore its interactions within p53-mediated pathways.
Validation of the antibody typically includes testing for specificity via knockout cell lines or siRNA knockdown. High-quality p53DINP1 antibodies are critical for understanding its dual role in promoting survival under mild stress or apoptosis under severe damage, offering insights into therapeutic strategies targeting p53-related diseases.
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