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产品描述：人干细胞因子/肥大细胞生长因子(SCF/MGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的KITLG含量。KITLG 即干细胞因子，在造血、生殖、黑素形成等生理过程中起重要作用。其通过与受体 KIT 结合，激活下游信号通路，调控细胞的增殖、分化和存活。当前，KITLG 在肿瘤、血液疾病等领域的研究机制是探索其在疾病发生发展中的作用及潜在治疗价值。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于体外研究SCF/MGF在造血系统疾病、过敏反应、皮肤病变等领域的表达调控机制，或用于细胞因子释放实验、药物干预研究及生物制剂质量控制等科研场景，为探索SCF相关信号通路提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C kit ligand ELISA Kit; C-kit ligand ELISA Kit; Ckit ligand ELISA Kit; DCUA ELISA Kit; DFNA69 ELISA Kit; DKFZp686F2250 ELISA Kit; familial progressive hyperpigmentation 2 ELISA Kit; FPH2 ELISA Kit; FPHH ELISA Kit; KIT ligand ELISA Kit; Kitl ELISA Kit; KITLG ELISA Kit; KL 1 ELISA Kit; KL1 ELISA Kit; Mast cell growth factor ELISA Kit; MGF ELISA Kit; MGF stem cell factor ELISA Kit; SCF ELISA Kit; SCF_HUMAN ELISA Kit; SF ELISA Kit; SHEP7 ELISA Kit; sKITLG ELISA Kit; Soluble KIT ligand ELISA Kit; Steel factor ELISA Kit; steel, mouse, homolog of ELISA Kit; Stem cell factor ELISA Kit; Stem cell factor precursor ELISA Kit
缩写：KITLG
Uniprot No.：P21583
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of human SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-107 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.6242.5222.5732.461151.7961.8971.8471.7357.51.2811.1881.2351.1233.750.7390.7130.7260.6141.80.4290.4040.4170.3050.90.2770.2950.2860.1740.450.2150.2120.2140.10200.1110.1130.112 | ||||||
货期：3-5 working days
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