| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | COREST; Nr2b2; RCOR; Rcor1; Rxrb;;REST corepressor 1 |
| WB Predicted band size | Calculated MW: 53 kDa ; Observed MW: 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human REST corepressor 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于CoREST抗体的参考文献，按研究领域分类简要概括：
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1. **文献名称**：*Co-repressor-dependent silencing of chromosomal regions encoding neuronal genes*
**作者**：You, A., Tong, J.K., Grozinger, C.M., Schreiber, S.L.
**摘要**：本研究利用CoREST抗体通过ChIP实验，揭示了CoREST与REST转录因子及组蛋白去乙酰化酶(HDAC)协同作用，沉默神经元基因在非神经细胞中的表达，阐明了其在染色质修饰中的核心机制。
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2. **文献名称**：*Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase*
**作者**：Yang, M., Gocke, C.B., Luo, M.
**摘要**：通过CoREST抗体的免疫共沉淀分析，作者解析了LSD1-CoREST复合物的晶体结构，证明CoREST作为支架蛋白介导LSD1对核小体组蛋白H3K4的去甲基化活性，为靶向表观遗传治疗提供结构基础。
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3. **文献名称**：*CoREST is a component of the functional LSD1/HDAC complex required for hematopoietic differentiation*
**作者**：Lee, M.G., Wynder, C., Schmidt, D.M., et al.
**摘要**：该研究在造血干细胞中通过CoREST抗体的Western blot和免疫荧光实验，证实CoREST与LSD1/HDAC形成复合物，调控关键分化基因的沉默，缺失CoREST会导致造血分化异常。
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**备注**：以上文献发表于2000-2010年间，集中于CoREST复合体的分子机制研究。如需近年应用类文献(如癌症或疾病模型)，建议补充关键词(如“CoREST antibody cancer”)进一步检索。
CoREST antibodies are essential tools for studying the CoREST corepressor complex, a critical regulator of gene expression and chromatin remodeling. The CoREST protein (also known as RCOR1) was initially identified as a binding partner of REST (RE1-Silencing Transcription Factor), a repressor of neuronal genes in non-neuronal cells. However, CoREST functions both in REST-dependent and independent contexts, forming multi-protein complexes with histone-modifying enzymes like LSD1 (lysine-specific histone demethylase 1) and HDAC1/2 (histone deacetylases 1/2). These complexes mediate transcriptional repression by removing activating histone marks (e.g., H3K4me2 via LSD1) and promoting chromatin condensation.
CoREST antibodies are widely used in techniques such as ChIP-seq, immunofluorescence, and Western blotting to investigate its role in development, differentiation, and disease. Studies reveal CoREST's involvement in stem cell maintenance, neuronal development, and oncogenesis. Dysregulation of CoREST complexes is linked to cancers (e.g., leukemia, neuroblastoma) and neurological disorders. Antibodies targeting CoREST help characterize its interaction networks, subcellular localization, and dynamic recruitment to specific genomic loci. Notably, CoREST's dual role in gene repression and activation (via context-dependent partnerships) underscores its functional complexity, driving demand for high-specificity antibodies. Recent work also explores CoREST inhibitors as potential therapeutics, highlighting the translational relevance of these research tools.
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