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产品描述：人整合素α-IIb(ITGA2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ITGA2B含量。ITGA2B 即整合素α2b，是血小板表面的重要糖蛋白。它可与β3 亚基形成复合物，在血小板聚集和血栓形成中起关键作用。目前围绕它开发抗血小板药物是热门研究方向，通过抑制其与纤维蛋白原结合，减少血小板聚集以防治血栓性疾病。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究血小板功能调控、抗血栓药物开发、肿瘤转移机制探索等科研领域，为相关基础研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：antigen CD41 ELISA Kit; BDPLT16 ELISA Kit; BDPLT2 ELISA Kit; CD41 ELISA Kit; CD41B ELISA Kit; form 2 ELISA Kit; GP2B ELISA Kit; GPalpha IIb ELISA Kit; GPIIb ELISA Kit; GT ELISA Kit; GTA ELISA Kit; HPA3 ELISA Kit; Integrin alpha 2b ELISA Kit; Integrin alpha IIb ELISA Kit; Integrin alpha-IIb light chain ELISA Kit; Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) ELISA Kit; ITA2B_HUMAN ELISA Kit; Itga2b ELISA Kit; ITGAB ELISA Kit; platelet fibrinogen receptor, alpha subunit ELISA Kit; platelet glycoprotein IIb of IIb/IIIa complex ELISA Kit; Platelet membrane glycoprotein IIb ELISA Kit; platelet specific antigen BAK ELISA Kit; PPP1R93 ELISA Kit
缩写：ITGA2B
Uniprot No.：P08514
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 93-107 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 90-103 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 81-92 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 88-100 | ||||||
回收率：
| The recovery of human ITGA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 83-94 | |||||
| EDTA plasma (n=4) | 104 | 94-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.2932.1442.2192.06251.3251.2741.3001.1432.50.8810.8670.8740.7171.250.5130.5230.5180.3610.6250.3460.3510.3490.1920.3120.2890.2800.2850.1280.1560.2120.2190.2160.05900.1590.1550.157 | |||||||
货期：3-5 working days
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