首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素9(IL-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL9含量。IL9是一种细胞因子，在免疫系统中发挥重要作用。它能调节多种免疫细胞的功能，与过敏反应、自身免疫疾病等发病机制相关。目前对其研究主要聚焦于揭示它在免疫调节网络中的作用机制，以探索基于IL9靶点的疾病治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外研究IL-9在免疫调节、炎症反应中的分子机制，或评估疾病模型中细胞因子表达动态变化，为免疫学、肿瘤微环境及自身免疫性疾病等领域的科研实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cytokine P40 ELISA Kit; Homolog of mouse T cell and mast cell growth factor 40 ELISA Kit; HP40 ELISA Kit; IL 9 ELISA Kit; IL-9 ELISA Kit; Il9 ELISA Kit; IL9_HUMAN ELISA Kit; Interleukin 9 ELISA Kit; Interleukin-9 ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Megakaryoblast growth factor ELISA Kit; P40 ELISA Kit; p40 cytokine ELISA Kit; p40 T cell and mast cell growth factor ELISA Kit; T cell growth factor 3 ELISA Kit; T cell growth factor p40 ELISA Kit; T-cell growth factor P40 ELISA Kit; TCGF 3 ELISA Kit
缩写：IL9
Uniprot No.：P15248
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-86 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4032.4982.4512.3065001.5201.5681.5441.3992500.8450.8980.8720.7271250.5230.5680.5460.40162.50.3650.3670.3660.22131.20.2820.2710.2770.13215.60.2210.2190.2200.07500.1430.1460.145 | ||||||
货期：3-5 working days
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