| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | prepl; Prolyl endopeptidase-like;;PREPL |
| WB Predicted band size | Calculated MW: 84 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PREPL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PREPL抗体的3篇代表性文献的简要信息整理：
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1. **文献名称**：*PREPL deficiency: delineation of the phenotype and development of a diagnostic blood test*
**作者**：Regal L, et al.
**摘要**：本研究首次建立了通过Western blot检测外周血白细胞中PREPL蛋白表达的方法，使用特异性抗体确认了先天性肌无力综合征患者中PREPL基因缺失导致的蛋白完全缺失，为临床诊断提供了可靠工具。
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2. **文献名称**：*The role of PREPL in cellular protein trafficking and metabolic regulation*
**作者**：Jaeken J, et al.
**摘要**：通过免疫荧光和免疫组化技术，研究团队利用PREPL抗体揭示了该蛋白在神经元和内分泌组织中的亚细胞定位，发现其与囊泡运输相关蛋白的相互作用，提示其在神经递质释放中的调控作用。
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3. **文献名称**：*Mouse models of PREPL deficiency exhibit growth retardation and metabolic alterations*
**作者**：Chabrol B, et al.
**摘要**：构建PREPL基因敲除小鼠模型，采用特异性抗体验证了多组织中PREPL蛋白的缺失，并发现模型小鼠出现脂肪代谢异常和生长迟缓，为研究人类PREPL相关疾病提供了动物模型基础。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以关键词“PREPL antibody”或“PREPL deficiency”检索最新研究，并优先选择近五年内发表的论文以获取前沿进展。
PREPL (Prolyl Endopeptidase-Like) is a serine hydrolase belonging to the prolyl oligopeptidase family. Although structurally similar to prolyl endopeptidase (PREP), PREPL lacks enzymatic activity toward typical peptide substrates. It is highly expressed in the brain, particularly in the striatum, and plays a role in vesicular trafficking, neurotransmitter release, and lysosomal function. PREPL interacts with proteins such as AP-3 complex subunits and CLIPR-59. influencing synaptic vesicle recycling and cytoskeletal dynamics.
Antibodies targeting PREPL are critical tools for studying its expression, localization, and molecular interactions. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PREPL's role in cellular processes. Research has linked PREPL deficiencies to human disorders, including hypotonia-cystinuria syndrome (HCS), a recessive condition caused by deletions in the SLC3A1-PREPL chromosomal region. PREPL antibodies also aid in diagnosing such genetic disorders and exploring their pathophysiology, particularly neurological and neuromuscular manifestations.
Current studies focus on PREPL's involvement in neurotransmitter regulation and its potential connection to developmental delays or metabolic disruptions. These antibodies remain essential for unraveling PREPL's biological significance and therapeutic potential.
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