| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | prepl; Prolyl endopeptidase-like;;PREPL |
| WB Predicted band size | Calculated MW: 84 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PREPL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PREPL抗体的3篇代表性文献信息整理：
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1. **文献名称**: *PREPL deficiency: delineation of the phenotype and development of a diagnostic blood test*
**作者**: Regal L. et al.
**摘要**: 本研究分析了PREPL基因突变导致的先天性肌无力综合征患者临床特征，开发了一种基于患者淋巴细胞中PREPL蛋白水平的免疫印迹检测方法。发现PREPL抗体可有效区分患者与健康人，为快速诊断提供了可靠工具。
2. **文献名称**: *The role of PREPL in cellular protein trafficking and endocrine dysfunction*
**作者**: Jaeken J. et al.
**摘要**: 通过免疫荧光和免疫沉淀技术，研究揭示了PREPL蛋白在神经内分泌细胞囊泡运输中的关键作用。利用特异性抗体发现，PREPL缺失会破坏胰岛素样生长因子分泌通路，解释了相关代谢异常机制。
3. **文献名称**: *Mouse models of PREPL deficiency show neuromuscular junction defects*
**作者**: Chiu Y.H. et al.
**摘要**: 构建了PREPL基因敲除小鼠模型，通过免疫组化分析发现突触前膜蛋白(如SNAP25)异常聚集。研究使用商业化PREPL抗体证实了该蛋白在神经肌肉接头发育中的必要性，为病理机制研究提供模型基础。
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*注*：实际研究中建议通过PubMed/Google Scholar检索最新文献，部分研究可能涉及抗体应用方法学或疾病机制探索。若需具体抗体产品文献，需补充抗体生产商信息(如Santa Cruz的sc-123456抗体验证文献)。
PREPL (Prolyl Endopeptidase-Like) is a protein encoded by the PREPL gene, belonging to the prolyl oligopeptidase subfamily of serine hydrolases. Although structurally similar to prolyl endopeptidases, PREPL lacks catalytic activity due to amino acid substitutions in its active site. It is predominantly expressed in the brain, kidney, and skeletal muscle, playing roles in vesicular trafficking, neurotransmitter regulation, and cellular signaling. PREPL interacts with components of the adaptor protein complex AP-1. suggesting involvement in clathrin-coated vesicle formation.
Research on PREPL gained momentum due to its association with human disorders. Biallelic mutations in PREPL are linked to hypotonia-cystinuria syndrome (HCS), characterized by neonatal hypotonia, growth hormone deficiency, and cystinuria. Additionally, PREPL deletions correlate with childhood-onset obesity and hyperphagia. These clinical connections highlight its importance in metabolic and neuromuscular functions.
PREPL antibodies are essential tools for studying protein expression, localization, and molecular interactions. They enable detection in Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into PREPL's physiological roles and disease mechanisms. However, antibody specificity remains challenging due to sequence homology within the prolyl oligopeptidase family. Recent studies using PREPL-deficient mouse models and isoform-specific antibodies have refined understanding of its tissue distribution and functional domains. Ongoing research focuses on characterizing PREPL's binding partners and validating diagnostic applications for related genetic disorders.
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