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Human Interleukin 4,IL-4 ELISA KIT

  • 中文名： 人白介素4(IL-4)ELISA Kit
货号: IPD-E04633h
Price： ￥2500
数量：
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  • 产品描述：
    人白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL4含量。IL4是一个在免疫学领域备受关注的靶点。它是一种细胞因子，在调节免疫反应、促进B细胞增殖和分化等方面发挥重要作用。研究机制主要围绕IL4与其受体结合，激活下游信号通路，影响细胞的生长、分化及功能，有望为免疫相关疾病治疗提供方向。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。适用于基础医学研究中Th1/Th2免疫平衡机制探索、炎症模型动态监测或药物干预效果评价等场景，例如评估哮喘动物模型中的细胞因子表达谱，或筛选调节IL-4分泌的化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    B cell growth factor 1 ELISA Kit; B cell IgG differentiation factor ELISA Kit; B Cell Stimulatory Factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BCGF 1 ELISA Kit; BCGF1 ELISA Kit; Binetrakin ELISA Kit; BSF-1 ELISA Kit; BSF1 ELISA Kit; IGG1 induction factor ELISA Kit; IL 4 ELISA Kit; IL-4 ELISA Kit; IL4 ELISA Kit; IL4_HUMAN ELISA Kit; Il4e12 ELISA Kit; Interleukin 4 ELISA Kit; Interleukin 4 variant 2 ELISA Kit; Interleukin 4; isoform 1 ELISA Kit; Interleukin-4 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit; MGC79402 ELISA Kit; Pitrakinra ELISA Kit
  • 缩写：
    IL4
  • Uniprot No.：
    P05112
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    31.25 pg/ml - 2000 pg/ml
  • 灵敏度：
    7.8 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    88-100


    1:2

    Average %

    95


    Range %

    92-101


    1:4

    Average %

    97


    Range %

    93-100


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    90-95


    EDTA plasma (n=4)

    97

    92-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.807

    2.634

    2.721

    2.549


    1000

    1.855

    1.767

    1.811

    1.639


    500

    0.964

    1.028

    0.996

    0.824


    250

    0.526

    0.529

    0.528

    0.356


    125

    0.375

    0.351

    0.363

    0.192


    62.5

    0.306

    0.293

    0.299

    0.128


    31.25

    0.215

    0.204

    0.209

    0.038


    0

    0.168

    0.175

    0.172



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled IL-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Squirt bottle, manifold dispenser, or automated microplate washer.

      • Absorbent paper for blotting the microtiter plate.

      • 100ml and 500ml graduated cylinders.

      • Deionized or distilled water.


      • 货期：
        3-5 working days

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