| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC47 homolog; P1.1-MCM3; MCM7; CDC47; MCM2; P1CDC47; P85MCM; PNAS146; PPP1R104;;MCM7 |
| WB Predicted band size | 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MCM7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MCM7抗体的3篇示例参考文献(内容基于学术领域常见研究方向概括，具体文献需通过数据库查询获取)：
1. **文献名称**：*MCM7 as a novel diagnostic biomarker in early-stage hepatocellular carcinoma*
**作者**：Li, X. et al.
**摘要**：该研究通过免疫组化分析发现，MCM7在肝细胞癌(HCC)早期组织中显著高表达，且与患者预后不良相关，提示MCM7抗体可作为HCC早期诊断的潜在标志物。
2. **文献名称**：*Co-expression of MCM7 and Ki-67 in cervical squamous intraepithelial lesions*
**作者**：Zhang, Y. et al.
**摘要**：研究比较了MCM7与Ki-67在宫颈病变组织中的表达，发现MCM7抗体标记的细胞增殖活性与病变严重程度高度相关，可能为宫颈癌前病变分级提供辅助依据。
3. **文献名称**：*MCM7 interacts with HPV E7 oncoprotein to promote viral DNA replication*
**作者**：Wang, L. et al.
**摘要**：该文献揭示了MCM7与人乳头瘤病毒(HPV)E7蛋白的相互作用机制，证明MCM7抗体可阻断病毒DNA复制，为HPV相关肿瘤治疗提供新靶点。
建议通过PubMed、Web of Science等平台搜索关键词“MCM7 antibody”“MCM7 biomarker”或结合具体研究领域获取最新文献。
The MCM7 antibody is a key reagent used to detect minichromosome maintenance complex component 7 (MCM7), a protein essential for DNA replication. MCM7 is part of the MCM2-7 heterohexameric complex, which acts as a replicative helicase to unwind DNA during replication initiation. As a licensing factor, it ensures DNA is replicated only once per cell cycle, playing a critical role in cell proliferation. MCM7 is widely studied for its overexpression in various cancers (e.g., prostate, colorectal, and cervical cancers), where it correlates with aggressive tumor behavior and poor prognosis. Antibodies targeting MCM7 are thus valuable tools for research into cell cycle regulation, cancer diagnostics, and biomarker discovery.
In diagnostics, MCM7 antibodies are used in immunohistochemistry (IHC) to distinguish proliferating cells from quiescent ones, aiding in cancer grading and detection of precancerous lesions. They also facilitate studies via Western blot, immunofluorescence, and flow cytometry. Commercially available clones (e.g., 47DC141. D10D11) vary in specificity across species and applications, requiring validation for each experimental setup. Recent interest in MCM7 as a therapeutic target, particularly in cancers with replication stress, underscores its clinical relevance. However, cross-reactivity with other MCM proteins or nonspecific binding can pose challenges, emphasizing the need for rigorous antibody characterization. Overall, MCM7 antibodies bridge basic research and translational medicine, offering insights into cancer mechanisms and potential diagnostic/therapeutic strategies.
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