| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | COUP-TF1; EAR3; ERBAL3; NR2F1; NR2F2; Nuclear receptor subfamily 2 group F member 1; SVP44; TCFCOUP1; TFCOUP1; ;COUP TF1 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human COUP TF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于COUP-TF1(COUP Transcription Factor 1)抗体的模拟参考文献示例(注：文献为模拟生成，非真实存在，仅供格式参考)：
1. **文献名称**: "COUP-TF1 Antibody Specificity and Application in Developmental Biology Studies"
**作者**: Smith A, et al.
**摘要**: 本研究验证了COUP-TF1抗体在免疫组化(IHC)和Western blot(WB)中的特异性，证明其在小鼠胚胎组织中可特异性识别COUP-TF1蛋白，并揭示该蛋白在神经管发育中的动态表达模式。
2. **文献名称**: "COUP-TF1 as a Regulator of Tumor Angiogenesis: Insights from Antibody-Based Inhibition Assays"
**作者**: Lee JH, et al.
**摘要**: 通过使用高特异性COUP-TF1抗体，研究者发现COUP-TF1通过调控VEGF信号通路影响肿瘤血管生成，抗体阻断实验表明其可能成为抗血管生成治疗的潜在靶点。
3. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against Human COUP-TF1 for Clinical Diagnostics"
**作者**: Garcia R, et al.
**摘要**: 报道一种新型单克隆抗体的开发，该抗体在福尔马林固定石蜡包埋(FFPE)组织中表现出高亲和力，可用于临床样本(如乳腺癌)中COUP-TF1蛋白表达的定量分析，与患者预后显著相关。
4. **文献名称**: "COUP-TF1 Antibody Reveals Its Role in Hepatocyte Differentiation"
**作者**: Chen X, et al.
**摘要**: 利用COUP-TF1抗体进行染色质免疫沉淀(ChIP-seq)分析，发现其在肝细胞分化中直接调控关键代谢基因(如CYP3A4)，为肝脏疾病机制研究提供依据。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词如 *"COUP-TF1 antibody validation"* 或 *"NR2F1 antibody application"*(COUP-TF1的官方基因符号为NR2F1)。
COUP-TF1 (Chicken Ovalbumin Upstream Promoter Transcription Factor 1), also known as NR2F1. is a member of the nuclear receptor superfamily. It functions as a transcription factor regulating gene expression by binding to specific DNA sequences, often as a monomer or homodimer. COUP-TF1 plays critical roles in embryonic development, including neurogenesis, angiogenesis, and organogenesis. It is involved in cell fate determination, differentiation, and metabolic regulation, with activity modulated by post-translational modifications and interactions with co-regulators.
Antibodies targeting COUP-TF1 are essential tools for studying its expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from conserved regions of the human or mouse COUP-TF1 protein. They enable applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validated antibodies show specificity for COUP-TF1 over closely related family members like COUP-TF2 (NR2F2).
COUP-TF1 dysregulation has been linked to neurodevelopmental disorders (e.g., Bosch-Boonstra-Schaaf optic atrophy syndrome) and cancers, where it may act as an oncogene or tumor suppressor depending on context. Research using COUP-TF1 antibodies has elucidated its role in stem cell maintenance, metabolic reprogramming, and disease pathways. Proper validation via knockout controls or siRNA knockdown is crucial due to potential cross-reactivity with paralogs or splice variants.
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