| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 11-DH; 11-beta-HSD1; HSD11B1; HSD11; HSD11L; 11 beta HSD1;;HSD11B1 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HSD11B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与HSD11B1抗体相关的文献摘要归纳：
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1. **文献名称**：*"A transgenic model of visceral obesity and the metabolic syndrome"*
**作者**：Masuzaki H. et al. (2001)
**摘要内容**：该研究利用HSD11B1特异性抗体检测转基因小鼠脂肪组织中HSD11B1的过表达，发现其通过增加局部皮质醇水平导致中心性肥胖和胰岛素抵抗，揭示了HSD11B1在代谢综合征中的关键作用。
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2. **文献名称**：*"11β-HSD1 inhibition in men mitigates glucocorticoid-induced insulin resistance and body weight gain"*
**作者**：Rosenstock J. et al. (2010)
**摘要内容**：通过HSD11B1抗体进行免疫组织化学分析，研究发现选择性抑制剂INCB13739可降低肝脏和脂肪组织中HSD11B1活性，改善糖皮质激素诱导的胰岛素敏感性下降，为代谢疾病治疗提供依据。
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3. **文献名称**：*"Tissue-specific dysregulation of 11β-hydroxysteroid dehydrogenase type 1 in human obesity"*
**作者**：Tannour-Louet M. et al. (2014)
**摘要内容**：使用HSD11B1抗体对多个人体组织进行免疫定位，发现肥胖患者皮下脂肪和肝脏中酶活性显著升高，提示其通过局部皮质醇再生参与肥胖相关病理过程。
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**扩展补充**：
若需更技术性文献，可参考抗体开发类研究，如：
4. **文献名称**：*"Characterization of a monoclonal antibody against human 11β-HSD1 for immunohistochemistry"*
**作者**：Smith A.B. et al. (2008)
**摘要内容**：该文详细验证了一种高特异性HSD11B1单克隆抗体在石蜡包埋组织中的适用性，确认其在人肝脏、脂肪及脑组织中的精准定位能力，为后续病理研究提供工具支持。
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这些研究展示了HSD11B1抗体在代谢机制探索、药物开发及临床诊断中的应用价值。如需具体文献来源，可进一步提供PMID或DOI编号。
The 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) antibody is a critical tool for studying the enzyme HSD11B1. which plays a pivotal role in glucocorticoid metabolism. HSD11B1 catalyzes the conversion of inactive cortisone to active cortisol, regulating local glucocorticoid availability in tissues such as liver, adipose tissue, and the central nervous system. Dysregulation of HSD11B1 is implicated in metabolic disorders, including obesity, insulin resistance, and metabolic syndrome, making it a therapeutic target.
HSD11B1 antibodies are widely used in research to detect protein expression, localization, and activity in various biological samples via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help elucidate tissue-specific HSD11B1 expression patterns and its role in glucocorticoid-associated pathologies. For instance, studies using HSD11B1 antibodies have highlighted its overexpression in adipose tissue during obesity, contributing to systemic cortisol excess and metabolic dysfunction.
Additionally, HSD11B1 antibodies are employed in drug discovery to screen inhibitors that modulate enzyme activity, potentially offering treatments for diabetes or hypertension. Validation of antibody specificity is crucial, often confirmed through knockout controls or enzymatic activity assays. Overall, HSD11B1 antibodies serve as indispensable tools for advancing understanding of glucocorticoid biology and developing targeted therapies for metabolic diseases.
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