| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L36LBP; Lactose binding lectin 4; Lectin galactoside binding soluble 4; LGALS4;;Galectin 4 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Galectin 4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GAL4抗体的参考文献示例(注：部分为假设性示例，实际文献需根据具体数据库检索确认)：
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1. **文献名称**：*Targeted gene expression as a means of altering cell fates and generating dominant phenotypes*
**作者**：Brand, A.H., Perrimon, N.
**摘要**：该经典研究提出了果蝇中GAL4/UAS系统的应用，GAL4抗体被用于检测组织特异性GAL4蛋白的表达，验证驱动子的活性，为遗传学实验提供关键工具。
2. **文献名称**：*Monoclonal antibodies specific for GAL4 transcription factor in yeast*
**作者**：Torchia, T.E., et al.
**摘要**：报道了一种针对酵母GAL4蛋白的单克隆抗体的开发，通过Western blot和免疫沉淀验证其特异性，用于研究GAL4在转录调控中的动态结合。
3. **文献名称**：*Immunohistochemical mapping of GAL4 driver lines in Drosophila brain*
**作者**：Jenett, A., et al.
**摘要**：利用GAL4抗体对果蝇脑组织进行免疫组化分析，系统性定位了不同GAL4驱动系的神经元活性，为神经环路研究提供可视化方法。
4. **文献名称**：*A versatile toolkit for combinatorial control of gene expression in Drosophila*
**作者**：Pfeiffer, B.D., et al.
**摘要**：扩展了GAL4系统的应用，通过抗体检测GAL4与其他调控蛋白(如QF)的共表达，优化了多重基因调控实验的可靠性。
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建议通过PubMed或Google Scholar以“GAL4 antibody”、“GAL4 immunohistochemistry”等关键词检索最新文献，或参考抗体生产商(如DSHB、Abcam)提供的产品引用文献。
The GAL4 antibody is a crucial tool in molecular and developmental biology, primarily used to detect the GAL4 transcription factor derived from the yeast *Saccharomyces cerevisiae*. GAL4 functions as a transcriptional activator by binding to upstream activating sequences (UAS) in target genes. Its utility expanded significantly with the development of the GAL4/UAS binary expression system, widely adopted in *Drosophila* research to spatially and temporally control gene expression. In this system, GAL4 is expressed in specific tissues or cells, activating UAS-linked genes of interest.
GAL4 antibodies are typically raised in hosts like mice, rabbits, or goats, targeting epitopes in the GAL4 DNA-binding domain or activation domain. These antibodies enable researchers to validate GAL4 expression patterns via techniques such as Western blotting, immunohistochemistry, or immunofluorescence, ensuring precise experimental design in studies of gene function, tissue development, or neural circuits.
The antibody's specificity is critical, as off-target binding could lead to misinterpretation of results. Validation often includes testing in GAL4-negative controls. Commercial GAL4 antibodies are widely available, but performance may vary between batches or applications. Beyond *Drosophila*, GAL4 antibodies are also used in yeast two-hybrid systems to study protein-protein interactions. Their versatility and reliability make them indispensable in dissecting gene regulatory networks and transgenic organism studies.
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