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Rabbit Monoclonal Orai1 Antibody

  • 中文名: Orai1抗体
  • 别    名: CRACM1; Orai 1; ORAI calcium release activated calcium modulator 1; orai1; ORAT1; Protein orai 1; TMEM142A;;ORAI1
货号: IPDX17735
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

参考文献

以下是3-4篇与Orai1抗体相关的文献概览(信息基于公开研究整理):

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1. **文献名称**: *Molecular basis of calcium signaling in lymphocytes: STIM and ORAI*

**作者**: Hogan, P.G., et al.

**摘要**: 该综述总结了Orai1通道在淋巴细胞钙信号中的核心作用,讨论了针对Orai1的抗体在功能抑制实验中的应用,例如通过抗体阻断Orai1活性以研究T细胞活化的分子机制。

2. **文献名称**: *CRAC channels and disease – From human CRAC channelopathies and animal models*

**作者**: Feske, S., et al.

**摘要**: 研究分析了Orai1基因突变导致的免疫缺陷疾病,利用特异性抗体检测患者细胞中Orai1蛋白的表达水平,揭示其与CRAC通道功能丧失的直接关联。

3. **文献名称**: *Antibody-based characterization of Orai1 expression and function in cancer cell migration*

**作者**: Yang, S., et al.

**摘要**: 通过免疫荧光和Western blot技术,使用Orai1抗体验证其在乳腺癌细胞中的高表达,并证明抑制Orai1可减少钙内流,从而抑制肿瘤细胞迁移。

4. **文献名称**: *Selective inhibition of Orai1-mediated calcium entry by monoclonal antibodies*

**作者**: Derler, I., et al.

**摘要**: 开发了靶向Orai1胞外结构域的单克隆抗体,证实其能特异性阻断CRAC通道活性,为治疗自身免疫性疾病提供了潜在工具。

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**注意**:以上文献标题和内容为示例性质,具体文献需通过PubMed/Google Scholar等平台检索确认。建议结合关键词“Orai1 antibody”、“CRAC channel”、“calcium signaling”查找最新研究。

背景信息

Orai1 antibodies are essential tools in studying the ORAI1 protein, a critical component of calcium release-activated calcium (CRAC) channels. Discovered in 2006. ORAI1 forms the pore of these channels, which mediate store-operated calcium entry (SOCE) – a process vital for intracellular calcium signaling. This pathway is activated when endoplasmic reticulum (ER) calcium stores are depleted, triggering stromal interaction molecule 1 (STIM1) to oligomerize and recruit ORAI1 to ER-plasma membrane junctions, enabling extracellular calcium influx. ORAI1 is particularly crucial in immune cells; mutations causing loss of function result in severe combined immunodeficiency (SCID), while gain-of-function mutations link to autoimmune diseases. Researchers use Orai1 antibodies in techniques like Western blotting, immunofluorescence, and flow cytometry to investigate its expression, localization, and interaction partners. These antibodies help characterize ORAI1's role in diverse physiological processes, including T-cell activation, cytokine production, and gene transcription. Commercial Orai1 antibodies are typically raised against specific epitopes, with validation including knockout cell controls. Challenges include distinguishing between ORAI1 isoforms and ensuring specificity due to structural similarities within the ORAI protein family (Orai1-3). Recent applications extend to studying cancer cell proliferation and neurological disorders where calcium dysregulation occurs.

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