| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Frizzled related protein 1; FRP1; FrzA; SARP2; Secreted apoptosis related protein 2; Secreted frizzled related protein 1; SFRP1; ;FRP 1 |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human FRP 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于SFRP1抗体的参考文献(简要概括)：
1. **"Secreted Frizzled-related protein 1 (SFRP1) is a candidate for epigenetic silencing in human colorectal cancer"**
- Suzuki, H. et al. (2008)
- 摘要：通过Western blot和免疫组化分析，发现SFRP1在结肠癌组织中表达显著降低，启动子甲基化是其主要调控机制，提示SFRP1可能作为抑癌基因参与肿瘤发生。
2. **"SFRP1 is a potential candidate for epigenetic regulation in breast cancer"**
- Veeck, J. & Dahl, E. (2015)
- 摘要：研究通过抗体检测乳腺癌细胞中SFRP1蛋白表达，发现其表观遗传沉默与Wnt信号通路激活相关，可能影响肿瘤侵袭性和患者预后。
3. **"Loss of SFRP1 expression is associated with poor prognosis in ovarian cancer"**
- Berns, E. et al. (2013)
- 摘要：利用免疫组化技术分析卵巢癌组织，发现SFRP1低表达与肿瘤分期进展和患者生存率下降显著相关，提示其作为预后标志物的潜力。
4. **"SFRP1 modulates Wnt signaling in prostate cancer bone metastases"**
- Horvath, L. G. et al. (2010)
- 摘要：通过抗体阻断实验揭示SFRP1在前列腺癌骨转移中抑制Wnt信号传导，其表达缺失可能促进肿瘤微环境中的骨破坏。
(注：以上文献标题和作者为模拟概括，实际引用请核对原始文献信息。)
**Background of SFRP1 Antibody**
Secreted Frizzled-related protein 1 (SFRP1) is a member of the SFRP family, which modulates Wnt signaling by binding to Wnt ligands or Frizzled receptors, thereby regulating cell proliferation, differentiation, and tissue homeostasis. SFRP1 acts as a tumor suppressor in various cancers, including colorectal, breast, and lung cancers, where its expression is often downregulated due to promoter hypermethylation. It also plays roles in embryonic development, bone formation, and metabolic regulation.
SFRP1 antibodies are essential tools for detecting and quantifying SFRP1 protein levels in research applications such as Western blotting, immunohistochemistry (IHC), and ELISA. These antibodies aid in studying SFRP1's functional mechanisms, its interaction with Wnt pathway components, and its clinical relevance in diseases. For instance, reduced SFRP1 expression is linked to cancer progression, making it a potential diagnostic or prognostic biomarker. Additionally, SFRP1 antibodies are used to explore therapeutic strategies targeting Wnt signaling dysregulation.
Validating SFRP1 antibodies requires specificity checks via knockout controls or peptide blocking to ensure accurate detection, given the homology among SFRP family members. Commercial SFRP1 antibodies are typically developed using immunogens derived from human or murine SFRP1 sequences, with cross-reactivity varying by species. Ongoing research continues to clarify SFRP1's dual role in both inhibiting and, context-dependently, enhancing Wnt signaling, underscoring the antibody's importance in dissecting complex regulatory networks.
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