| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcineurin A1; CalcineurinA; CALN; CALNA 1; CALNA; CALNA1; CAM PRP; CNA alpha; CCN1;;PPP3CA |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PPP3CA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Calcineurin A抗体相关的代表性文献(人工模拟示例，非真实文献)：
1. **文献名称**: "Calcineurin A Isoform-Specific Antibodies Reveal Differential Subcellular Localization in Neurons"
**作者**: Smith J, et al.
**摘要**: 该研究开发了针对Calcineurin Aα和Aβ亚型的特异性抗体，通过免疫荧光和Western blot技术，揭示了二者在小鼠海马神经元中的差异亚细胞分布，提示其可能参与不同的信号通路调控。
2. **文献名称**: "Anti-Calcineurin Antibody Validation for Cardiac Hypertrophy Studies"
**作者**: Li X, et al.
**摘要**: 文章系统验证了商业来源的Calcineurin A抗体的特异性，通过siRNA敲低实验和心肌肥厚模型，证明该抗体可有效检测心脏组织中Calcineurin A的表达上调，并关联其与病理性心肌肥厚的分子机制。
3. **文献名称**: "Calcineurin-NFAT Signaling in T Cells: A Flow Cytometry Approach Using Phospho-Specific Antibodies"
**作者**: Garcia-Rivera D, et al.
**摘要**: 研究利用Calcineurin A抗体结合磷酸化NFAT检测，建立了流式细胞术分析T细胞活化中Calcineurin活性的新方法，为免疫抑制药物(如环孢素A)的作用机制提供了直接实验依据。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索确认。建议使用关键词"Calcineurin A antibody validation"或"Calcineurin A immunodetection"查找最新应用文献。
Calcineurin A (CnA) is the catalytic subunit of calcineurin, a calcium/calmodulin-dependent serine/threonine protein phosphatase (PP2B). Composed of a regulatory subunit (CnB) and a catalytic subunit (CnA), calcineurin plays critical roles in calcium-mediated signaling pathways. It is widely expressed in tissues, particularly in the brain, immune cells, and muscle. Upon calcium influx, calmodulin binds to CnA, displacing an autoinhibitory domain to activate phosphatase activity. Calcineurin regulates diverse cellular processes, including T-cell activation (via NFAT dephosphorylation), neuronal development, cardiac hypertrophy, and synaptic plasticity.
Antibodies targeting Calcineurin A are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). These antibodies typically recognize specific isoforms (CnAα, CnAβ, or CnAγ) or conserved regions across isoforms, depending on epitope design. Validation often includes knockout controls or phosphatase activity assays to confirm specificity.
Research applications focus on calcineurin's role in immune suppression (targeted by drugs like cyclosporine A), neurodegenerative diseases (e.g., Alzheimer's), and cardiovascular disorders. Cross-reactivity with homologous proteins or species variants should be carefully evaluated. High-quality CnA antibodies enable precise mechanistic insights into calcium signaling pathologies and therapeutic interventions.
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