首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的IL2含量。IL-2即白细胞介素-2，是一种免疫细胞因子。它由T细胞产生，在免疫系统中扮演重要角色，能促进T细胞、B细胞增殖分化，增强NK细胞活性等。通过与受体结合，激活下游信号通路来调节免疫反应，有望用于肿瘤、感染等疾病治疗。试剂盒检测范围为6.25 pg/mL-400 pg/mL，灵敏度为1.56 pg/mL。该产品适用于科研实验中评估大鼠免疫激活状态，例如研究炎症性疾病模型（如自身免疫疾病或感染模型）中细胞因子网络的变化规律，亦可应用于药物研发中免疫调节剂的效果评价，或通过分析组织匀浆样本探究局部免疫微环境的IL - 2分泌特征。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：IL2
Uniprot No.：P17108
种属：Rattus norvegicus (Rat)
样本类型：serum, urine, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.4892.4782.4842.2892001.9541.8961.9251.7301001.2921.2731.2831.088500.7970.7680.7830.588250.5030.4890.4960.30112.50.3640.3560.3600.1656.250.2760.2580.2670.07200.1980.1920.195 | ||||||
货期：3-5 working days
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