首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IL2含量。IL2是重要的细胞因子，在免疫调节中发挥关键作用。其背景上，是免疫系统抵御疾病的重要介质。研究机制方面，IL2可激活T细胞和NK细胞等免疫细胞，增强其杀伤活性，在肿瘤免疫治疗、自身免疫病等研究领域被广泛关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于动物模型研究、免疫机制探索、炎症相关疾病机制分析或疫苗效力评估等科研场景，为探究IL - 2在免疫系统中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：IL2
Uniprot No.：O77620
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-95 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of rabbit IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 80-98 | ||||
| EDTA plasma (n=4) | 93 | 84-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4122.3202.3662.18110002.1742.0812.1281.9435001.8821.7431.8131.6282501.5211.4821.5021.3171251.0481.0441.0460.86162.50.6880.6540.6710.48631.250.3310.3080.3200.13500.1910.1790.185 | ||||||
货期：3-5 working days
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