| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Endoplasmin; GRP-94; gp96 homolog; HSP90B1; GRP 94; TRA1;;Hsp90 beta 1 |
| WB Predicted band size | Calculated MW: 92 kDa ; Observed MW: 94 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Hsp90 beta 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于GRP94抗体的代表性文献概览：
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1. **文献名称**：*GRP94 in Immune Disease: Molecular Mechanisms and Therapeutic Opportunities*
**作者**：Lee AS 等
**摘要**：探讨GRP94作为内质网分子伴侣在免疫细胞(如树突状细胞和B细胞)中的关键作用，揭示其通过调控Toll样受体和整合素信号通路参与自身免疫疾病(如类风湿性关节炎)的机制，提出靶向GRP94抗体治疗相关疾病的潜力。
2. **文献名称**：*Targeting GRP94 in Cancer: Antibody-Based Inhibition of Tumor Growth and Metastasis*
**作者**：Liu B, DeNardo DG
**摘要**：研究GRP94在肿瘤微环境中的促癌功能，发现其通过调节Wnt/β-catenin信号通路促进肿瘤血管生成。使用单克隆抗体阻断GRP94可显著抑制小鼠模型中黑色素瘤的生长和转移，提示其作为癌症治疗靶点的可行性。
3. **文献名称**：*GRP94-Specific Antibodies Disrupt Viral Protein Trafficking and Reduce Pathogen Infectivity*
**作者**：Zhang Y, Sun S
**摘要**：揭示GRP94在病毒(如登革热病毒和HCV)包膜蛋白折叠中的关键作用，开发特异性抗体干扰病毒蛋白的分泌，显著降低病毒颗粒感染力，为抗病毒治疗提供新策略。
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这些研究覆盖了GRP94抗体在疾病机制探索和靶向治疗中的应用，涉及癌症、免疫疾病和病毒感染领域。如需具体文献编号或实验细节，建议在PubMed或Web of Science中以“GRP94 antibody”和上述关键词组合检索。
GRP94 (Glucose-Regulated Protein 94), also known as HSP90B1 or gp96. is a member of the heat shock protein 90 (HSP90) family. It resides primarily in the endoplasmic reticulum (ER) and functions as a molecular chaperone, facilitating the folding and assembly of secreted and transmembrane proteins, including integrins, Toll-like receptors, and immunoglobulins. Unlike cytosolic HSP90 isoforms, GRP94 contains an ER-retention motif (KDEL) and plays a critical role in ER stress responses, calcium homeostasis, and the unfolded protein response (UPR).
Antibodies targeting GRP94 are essential tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate GRP94's involvement in diseases such as cancer, autoimmune disorders, and neurodegenerative conditions. GRP94 is often overexpressed in tumors, where it supports oncoprotein stability and tumor survival, making it a potential therapeutic target. Specific antibodies help distinguish GRP94 from other HSP90 family members (e.g., HSP90α/β) and assess its functional roles.
Research also explores GRP94 antibodies for therapeutic applications. Monoclonal antibodies blocking GRP94-client interactions or disrupting its chaperone activity have shown promise in preclinical cancer models. Additionally, GRP94’s role in antigen presentation links it to immune modulation, inspiring antibody-based strategies for vaccine development. These antibodies are typically validated for species reactivity (e.g., human, mouse) and application-specific performance to ensure specificity.
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