首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔白介素17(IL-17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17含量。IL-17 是重要的细胞因子，在免疫应答和炎症反应中起关键作用。它与自身免疫性疾病、感染性疾病等多种疾病相关。研究机制方面，主要聚焦其信号通路、与其他细胞因子相互作用等，以探索通过干预 IL-17 治疗相关疾病的方法。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于炎症模型构建、免疫应答机制研究、药物干预效果评价等科研场景，例如通过分析感染或自身免疫疾病模型中血清IL - 17水平变化，或探究特定组织内细胞因子表达特征，为免疫相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-17
Uniprot No.：G1SLF2
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 81-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 85-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of rabbit IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 85-100 | ||||
| EDTA plasma (n=4) | 95 | 84-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8602.7122.7862.67010002.2482.2122.2302.1145001.3921.3871.3901.2742500.7540.7290.7420.6261250.4560.4320.4440.32862.50.2750.2670.2710.15531.250.2130.1990.2060.09000.1190.1120.116 | ||||||
货期：3-5 working days
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