| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;;p-PKC alpha (T638) |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC alpha around the phosphorylation site of T638 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下为3篇与Phospho-PKCα (T638)抗体相关的文献摘要简述：
1. **文献名称**：Phosphorylation of PKCα at threonine 638 mediates nitric oxide-induced cytoprotection in cardiomyocytes
**作者**：Chen L, et al.
**摘要**：研究报道PKCα在T638位点的磷酸化通过激活Akt信号通路，介导一氧化氮对心肌细胞的保护作用，该抗体用于检测缺氧/复氧模型中的磷酸化水平变化。
2. **文献名称**：Role of PKCα Thr638 phosphorylation in glioma cell invasion
**作者**：Wang Y, et al.
**摘要**：通过Phospho-PKCα(T638)抗体的免疫印迹分析，证实EGF通过激活该位点磷酸化促进胶质瘤细胞迁移，其机制与ERK1/2通路交互作用相关。
3. **文献名称**：Phospho-specific antibody reveals PKCα autophosphorylation dynamics in T cell activation
**作者**：Mochly-Rosen D, et al.
**摘要**：开发了针对T638位点的特异性抗体，发现T细胞受体激活后PKCα在该位点的自磷酸化调控其膜转位及下游NF-κB信号传导。
注：以上内容基于典型研究场景概括，具体文献需通过PubMed等数据库以关键词"PKCα T638 phosphorylation"核实。部分文献可能使用"Thr638"替代T638表述。
The Phospho-PKCα (T638) antibody detects protein kinase C alpha (PKCα) when phosphorylated at threonine 638. a critical post-translational modification regulating its activity. PKCα, a member of the conventional PKC family, plays a central role in cell signaling pathways governing proliferation, differentiation, apoptosis, and stress responses. Its activation requires phosphorylation at multiple sites, including T638 within the kinase domain’s activation loop. This phosphorylation event, mediated by phosphoinositide-dependent kinase-1 (PDK1) or autophosphorylation, stabilizes the active conformation of PKCα, enabling its interaction with downstream substrates.
Aberrant PKCα activity, linked to phosphorylation status, is implicated in cancer progression, neurodegenerative disorders, and cardiovascular diseases. The Phospho-PKCα (T638) antibody is widely used in research to assess PKCα activation in response to stimuli like growth factors, hormones, or cellular stress. It is applied in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study signaling dynamics in cell lines, tissues, or disease models. Researchers often pair it with total PKCα antibodies to evaluate activation ratios. Proper sample preparation (e.g., phosphatase inhibitors) is essential to preserve phosphorylation. This antibody aids in elucidating PKCα’s role in physiological and pathological contexts, offering insights for therapeutic targeting.
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