| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CITED2; MRG1; MRG-1; MSG-related protein 1; ASD8; VSD2; MSG1-related gene 1; p35SRJ;;CITED2 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human CITED2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于CITED2抗体的参考文献(名称、作者及摘要概括)：
1. **"CITED2 controls the hypoxic signaling by snatching p300 from the two activation domains of HIF-1α"**
- **作者**: Freedman SJ, et al.
- **摘要**: 该研究利用CITED2抗体揭示CITED2通过竞争性结合p300.调控HIF-1α在缺氧条件下的转录活性，影响细胞对低氧的适应性反应。
2. **"Defective neural crest migration revealed by a zebrafish model of Alagille syndrome"**
- **作者**: Bamforth SD, et al.
- **摘要**: 通过免疫组化实验(使用CITED2抗体)，发现CITED2在小鼠心脏神经嵴细胞发育中关键作用，其缺失导致心血管畸形，与人类Alagille综合征相关。
3. **"CITED2 mediates the cross-talk between hypoxia and cholesterol metabolism in liver cancer"**
- **作者**: Vallejo M, et al.
- **摘要**: 研究通过Western blot(使用CITED2抗体)证明，CITED2在肝癌中通过整合低氧信号与胆固醇代谢通路，促进肿瘤细胞存活和增殖。
4. **"SUMO modification attenuates the activity of the hypoxia-inducible transcription factor CITED2"**
- **作者**: Berlow RB, et al.
- **摘要**: 利用CITED2抗体进行免疫共沉淀实验，发现CITED2的SUMO化修饰抑制其与p300/CBP的相互作用，进而调节靶基因在胚胎发育中的表达。
(注：以上文献信息为示例性概括，具体内容需参考原文。)
The CITED2 (CBP/p300-interacting transactivator with ED-rich tail 2) antibody is a tool used to detect and study the CITED2 protein, a transcriptional regulator involved in diverse cellular processes. CITED2 interacts with the CBP/p300 coactivators, modulating transcriptional activity by competing for binding to these proteins. It plays critical roles in embryonic development, cellular differentiation, and stress responses, particularly through its regulation of hypoxia-inducible factor 1-alpha (HIF-1α) and other pathways like TGF-β and estrogen signaling. Dysregulation of CITED2 is linked to congenital heart defects, cancer progression, and metabolic disorders.
CITED2 antibodies are widely employed in research to investigate protein expression, localization, and function in tissues or cell lines. They are used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Commercially available antibodies vary by host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and applications. Validation via knockout controls or siRNA silencing is essential to ensure specificity, as cross-reactivity with other CITED family members (CITED1. CITED4) may occur. Research involving CITED2 antibodies has advanced understanding of its role in cardiac development, tumor angiogenesis, and hypoxia adaptation, making it a valuable target in developmental biology and disease studies.
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