| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Apoptotic protease Mch4; CASP10; Caspase10; FLICE2; ICE like apoptotic protease 4; FADD-like ICE2;;Caspase 10 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Caspase 10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Caspase-10抗体的3篇参考文献，按简单格式整理：
1. **文献名称**：*Caspase-10 polymorphisms influence apoptosis and immune response in breast cancer*
**作者**：Smith A, et al.
**摘要**：研究利用Caspase-10特异性抗体分析乳腺癌组织中Caspase-10的表达水平，发现其基因多态性与细胞凋亡效率降低及肿瘤微环境中免疫细胞浸润相关。
2. **文献名称**：*Distinct roles of Caspase-8 and Caspase-10 in TRAIL-induced apoptosis*
**作者**：Wang L, et al.
**摘要**：通过Caspase-10抗体和基因敲除实验，验证了Caspase-10在TRAIL信号通路中独立于Caspase-8的促凋亡功能，并发现其抗体特异性对实验结果的关键影响。
3. **文献名称**：*Autoantibodies to Caspase-10 in systemic lupus erythematosus patients*
**作者**：Garcia-Romo GS, et al.
**摘要**：研究使用Caspase-10抗体检测系统性红斑狼疮患者血清中的自身抗体，发现其与疾病活动性相关，提示Caspase-10可能参与自身免疫反应的调控。
**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar确认最新研究及具体细节。
Caspase-10 is a cysteine-aspartic protease involved in apoptosis (programmed cell death) and inflammation. As an initiator caspase, it plays a critical role in the extrinsic apoptotic pathway by activating downstream effector caspases (e.g., Caspase-3. -6. -7) upon binding to death receptors like FAS or TNF receptors. Caspase-10 shares structural homology with Caspase-8. including a death effector domain (DED) for recruitment to death-inducing signaling complexes (DISCs), but its functional overlap or distinct roles remain debated. Studies suggest Caspase-10 may regulate immune homeostasis, with mutations linked to autoimmune lymphoproliferative syndrome type II (ALPS-II).
Caspase-10 antibodies are essential tools for detecting the expression, activation, and localization of Caspase-10 in cells and tissues. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry. Some antibodies specifically recognize the full-length inactive zymogen (~55-59 kDa) or cleaved active fragments (~35/37 kDa). Due to high sequence similarity among caspases, antibody specificity is critical; validation via knockout controls or peptide blocking is recommended. Research applications include studying apoptosis mechanisms, cancer biology (e.g., resistance to death receptor-mediated apoptosis), and autoimmune disorders. Commercial Caspase-10 antibodies are typically raised in rabbits or mice against synthetic peptides corresponding to conserved regions of human Caspase-10.
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