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Human Interleukin 12,IL-12/P40 ELISA KIT

  • 中文名： 人白介素12(IL-12/P40)ELISA Kit
货号: IPD-E04598h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素12(IL-12/P40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12B含量。IL12B是一种编码白细胞介素12亚单位的基因，具有广泛的生物活性，对T细胞和自然杀伤细胞有作用。其在Th1细胞发育中起关键作用，并与多发性硬化症等疾病相关。研究IL12B有助于理解其信号传导和免疫调节机制，为相关疾病的治疗提供新思路。试剂盒检测范围为0.156 ng/ml-10 ng/ml，灵敏度为0.039 ng/mL。适用于体外实验中对炎症反应机制、免疫调节通路及相关疾病模型的分子机制研究；为免疫学、分子生物学领域的实验室提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CLMF ELISA Kit; CLMF p40 ELISA Kit; CLMF2 ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; IL 12B ELISA Kit; IL-12 subunit p40 ELISA Kit; IL-12B ELISA Kit; IL12 p40 ELISA Kit; IL12B ELISA Kit; IL12B_HUMAN ELISA Kit; IMD28 ELISA Kit; IMD29 ELISA Kit; Interleukin 12 beta chain ELISA Kit; Interleukin 12 p40 ELISA Kit; Interleukin-12 subunit beta ELISA Kit; NK cell stimulatory factor chain 2 ELISA Kit; NKSF ELISA Kit; NKSF2 ELISA Kit
  • 缩写：
    IL12B
  • Uniprot No.：
    P29460
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml-10 ng/ml
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    90-98


    1:2

    Average %

    102


    Range %

    97-105


    1:4

    Average %

    89


    Range %

    84-92


    1:8

    Average %

    98


    Range %

    91-101


  • 回收率：

    The recovery of human IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    85-96


    EDTA plasma (n=4)

    103

    97-106


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.629

    2.589

    2.609

    2.475


    5

    1.664

    1.631

    1.648

    1.514


    2.5

    1.042

    1.087

    1.065

    0.931


    1.25

    0.688

    0.672

    0.680

    0.546


    0.625

    0.438

    0.444

    0.441

    0.307


    0.312

    0.283

    0.293

    0.288

    0.154


    0.156

    0.201

    0.214

    0.208

    0.074


    0

    0.135

    0.133

    0.134




  • 货期：
    3-5 working days

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