| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC42; CDC42Hs; G25K; TKS; MIG5; Ras like protein TC25;;p-Cdc42/Rac1 (S71) |
| WB Predicted band size | Calculated MW: 21 kDa ; Observed MW: 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cdc42 around the phosphorylation site of S71 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-Rac1/Cdc42(Ser71)抗体的3篇示例参考文献(注：以下内容为虚构示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*"Phosphorylation of Rac1 at Ser71 Modulates Neuronal Morphogenesis via PAK1 Signaling"*
**作者**：Zhang L, et al.
**摘要**：本研究揭示了Rac1在Ser71位点的磷酸化通过抑制PAK1结合调控神经元树突发育，利用Phospho-Rac1(Ser71)抗体验证了该修饰在脑组织中的表达模式及功能。
2. **文献名称**：*"A Novel Cdc42 Phosphorylation Site at Ser71 Regulates Epithelial Cell Polarity"*
**作者**：Chen X, et al.
**摘要**：通过开发特异性Phospho-Cdc42(Ser71)抗体，作者发现该位点的磷酸化由PKCα介导，并破坏Cdc42与极性蛋白Par6的相互作用，影响上皮细胞极性的建立。
3. **文献名称**：*"Development and Validation of a Phospho-Specific Antibody for Rac1/Cdc42(Ser71) in Cancer Invasion Studies"*
**作者**：Kimura T, et al.
**摘要**：本文报道了一种高特异性Phospho-Rac1/Cdc42(Ser71)抗体的制备方法，并通过质谱和功能实验验证其在乳腺癌细胞侵袭模型中的应用，证明该磷酸化与转移能力相关。
4. **文献名称**：*"Ser71 Phosphorylation of Rac1 by Akt Promotes Cell Survival under Oxidative Stress"*
**作者**：Wang Y, et al.
**摘要**：研究利用Phospho-Rac1(Ser71)抗体证实Akt激酶通过磷酸化Rac1的Ser71位点抑制其促凋亡活性，揭示了该修饰在氧化应激中的保护机制。
**提示**：实际文献需通过PubMed、Google Scholar等平台以关键词“Phospho-Rac1 Ser71”“Phospho-Cdc42 Ser71”“antibody validation”等检索，并筛选涉及抗体开发、功能验证或机制研究的论文。
The Phospho-Rac1/Cdc42(Ser71) antibody is designed to detect Rac1 and Cdc42 proteins phosphorylated at serine 71. a post-translational modification linked to their functional regulation. Rac1 and Cdc42 belong to the Rho family of small GTPases, which act as molecular switches controlling cytoskeletal dynamics, cell motility, and intracellular signaling. Their activity is typically governed by GTP/GDP binding cycles, but phosphorylation has emerged as an alternative regulatory mechanism.
Phosphorylation at Ser71 in Rac1/Cdc42 is associated with altered interactions with downstream effectors or guanine nucleotide exchange factors (GEFs), potentially modulating their role in processes like cell migration, polarity, and vesicular trafficking. This modification has been implicated in pathological contexts, including cancer metastasis and neurological disorders, where aberrant Rac1/Cdc42 signaling contributes to disease progression.
The antibody is widely used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to study the activation status and subcellular localization of phosphorylated Rac1/Cdc42 in response to stimuli such as growth factors, cytokines, or cellular stress. Researchers employ it to explore signaling pathways involving kinases like PAK1 or AKT, which may mediate Ser71 phosphorylation. Specificity is often validated using phosphorylation-blocking peptides or cells lacking Rac1/Cdc42 expression. Its applications span cancer biology, immunology, and neuroscience, offering insights into how post-translational modifications fine-tune GTPase activity in health and disease.
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