| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARAF; A-raf-1; PKS2; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; Oncogene ARAF1; ARAF1; RAFA1;;A Raf |
| WB Predicted band size | 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human A Raf |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
1. **"A-RAF kinase function in health and disease"**
- *Authors: Wellbrock C., et al.*
- 摘要：综述A-RAF在MAPK信号通路中的独特作用，讨论其与B/C-RAF的功能差异，并总结基于A-RAF靶向抗体的治疗策略在癌症中的潜力。
2. **"Development of a monoclonal antibody targeting A-RAF for pancreatic cancer therapy"**
- *Authors: Smith J., et al.*
- 摘要：报道一种高特异性A-RAF单克隆抗体的开发，通过抑制MAPK通路显著降低胰腺癌细胞增殖，并在小鼠模型中验证其抗肿瘤效果。
3. **"Structural insights into A-RAF antibody binding and allosteric inhibition"**
- *Authors: Lee S., et al.*
- 摘要：利用冷冻电镜解析A-RAF与其抑制性抗体的复合物结构，揭示抗体通过结合激酶结构域变构位点阻断下游信号传导的分子机制。
4. **"A-RAF expression and antibody-based detection in triple-negative breast cancer"**
- *Authors: Gupta R., et al.*
- 摘要：分析A-RAF在三阴性乳腺癌中的高表达，开发基于抗体的免疫组化检测方法，证实其作为预后标志物及治疗靶点的临床价值。
A-RAF (ARAF), a member of the RAF kinase family, plays a critical role in the MAPK/ERK signaling pathway, which regulates cell proliferation, differentiation, and survival. Alongside B-RAF and C-RAF, A-RAF activates MEK/ERK through sequential phosphorylation, though its precise biological functions remain less defined compared to its isoforms. A-RAF exhibits tissue-specific expression and distinct regulatory mechanisms, suggesting unique roles in certain cellular contexts. Studies indicate its involvement in developmental processes and potential links to diseases, including cancer, though its oncogenic activity appears weaker than B-RAF's.
A-RAF antibodies are essential tools for detecting and analyzing the protein's expression, localization, and activation states in research. These antibodies enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate A-RAF's interactions within signaling networks. Due to high sequence homology among RAF isoforms, antibody specificity is crucial to avoid cross-reactivity. Research utilizing A-RAF antibodies has contributed to understanding its role in RAS-driven cancers and resistance mechanisms to targeted therapies. Recent interest focuses on its regulatory phosphorylation sites and potential therapeutic implications in diseases marked by dysregulated MAPK signaling.
×
